| Main Title |
Molecular Regulation of the Induction of Cytochrome P-450E in the Estuarine Fish 'Fundulus heteroclitus'. |
| Author |
Kloepper-Sams, P. J. ;
|
| CORP Author |
Woods Hole Oceanographic Institution, MA. ;Massachusetts Inst. of Tech., Cambridge.;National Science Foundation, Washington, DC.;Public Health Service, Washington, DC.;Environmental Protection Agency, Washington, DC. |
| Publisher |
cFeb 89 |
| Year Published |
1989 |
| Report Number |
WHOI0-89-1; NSF-OCE83-10505 ;PHS-ES-4220; |
| Stock Number |
PB89-198204 |
| Additional Subjects |
Estuaries ;
Fishes ;
Regulation ;
Antibodies ;
Theses ;
Ribonucleic acid ;
Cytochrome P-450 ;
Enzyme induction ;
Fundulus heteroclitus ;
Xenobiotic metabolism
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB89-198204 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
226p |
| Abstract |
Regulation of induction of P450IA1 (P-450E) in teleosts was examined by investigating temporal relationships between P-450E protein, activity, and mRNA levels, and measuring protein and heme turnover in the teleost Fundulus heteroclitus. Monoclonal antibodies used for P-450E protein detection were specific in immunoblots for purified scup (Stenotomus chrysops) P-450E, a single band corresponding to P-450E in scup microsomal mixtures, and the xenobiotic-inducible orthologue in other fish including Fundulus. P-450E mRNA was measured by translation of total RNA, precipitation with anti-P-450E polyclonal antibodies and autoradiography, or by hybridization of RNA with a trout P450IA1 cDNA. P-450E and ethoxyresorufin O-deethylase activity rose coordinately after treatment with Beta-naphthoflavone, lagging behind mRNA increases by about 25 hours. mRNA levels declined rapidly, despite prolonged elevated protein and activity levels. In a dual label experiment, P-450E was precipitated from solubilized microsomes. The apoprotein was calculated to have a half-life of 32 to 43 hours, the heme moiety a longer half-life of 104 hours. These results support a hypothesis that transcriptional enhancement is involved in initial stages of P-450E induction, while other forms of control are important in maintenance of P-450E expression. The study addressed a specific chemico-biological interaction - the organism's biochemical response to a challenge by foreign compounds - which occurs in the marine environment. (Copyright (c) Pamela J. Kloepper-Sams, 1989.) |
| Supplementary Notes |
Sponsored in part by grants EPA-R-813155 and EPA-X-813567. Prepared in cooperation with Massachusetts Inst. of Tech., Cambridge. Sponsored by National Science Foundation, Washington, DC., Public Health Service, Washington, DC., and Environmental Protection Agency, Washington, DC. |
| NTIS Title Notes |
Doctoral thesis. |
| Category Codes |
47D; 57B |
| NTIS Prices |
PC A11/MF A01 |
| Primary Description |
100/00 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
925820676 |
| Origin |
NTIS |
| Type |
CAT |
