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OLS Field Name OLS Field Data
Main Title Improved Sea Urchin DNA-Based Embryo Growth Toxicity Test.
Author Jackim, E. ; Nacci, D. ;
CORP Author Environmental Research Lab., Narragansett, RI.
Year Published 1986
Report Number EPA/600/J-86/266;
Stock Number PB87-167144
Additional Subjects Toxicity ; Sea urchins ; Deoxyribonucleic acids ; Embryos ; Growth ; Fluorometers ; Aquatic biology ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB87-167144 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 7p
Abstract The article presents an improved DNA-based embryo toxicity test in which growth is measured as the increase in DNA during a 5-h postfertilization period. The test, using direct fluorometric determination of DNA rather than thymidine incorporation, is fast, sensitive, easy to conduct, and correlates well with other 48- and 96-h acute aquatic tests. The need for radioisotopes has been eliminated and data variability is substantially reduced. It is recommended that this test be incorporated into a battery of rapid tests used to estimate aquatic toxicity.
Supplementary Notes Pub. in Environmental Toxicology and Chemistry, v5 p561-567 1986.
NTIS Title Notes Journal article.
Title Annotations Reprint: Improved Sea Urchin DNA-Based Embryo Growth Toxicity Test.
PUB Date Free Form 1986
Category Codes 57Y; 57Z; 68G
NTIS Prices PC A02/MF A01
Primary Description 600/05
Document Type NT
Cataloging Source NTIS/MT
Control Number 715626597
Origin NTIS
Type CAT