Main Title |
Isolation of Mutants Temperature-Sensitive for Expression of the Transformed State from Chemically Transformed C3H/10Tl/2 Cells. |
Author |
Boreiko, Craig ;
Heidelberger, Charles ;
|
CORP Author |
University of Southern California, Los Angeles.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1980 |
Report Number |
EPA-R-805208; EPA-600/J-80-307; |
Stock Number |
PB81-232050 |
Additional Subjects |
Sensitivity ;
Embryos ;
Mice ;
Isolation ;
Temperature ;
Reprints ;
Fibroblasts ;
Cell lines
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-232050 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
17p |
Abstract |
58 MCA Cl 16 is an oncogenic methylocholanthrene-transformed variant of the non-transformed mouse embryo fibroblast cell line, C3H/10T1/2 Cl 8. Using two different protocols, they have isolated six temperature-sensitive mutants from N-methyl-N' - nitro N-nitrosoguanidine treated cultures of 58 MCA Cl 16. C3H/10T1/2 Cl 8, 58 MCA Cl 16 and the six mutant lines were characterized with respect to several properties associated with the transformed state: morphology, saturation density, anchorage independence, cell surface morphology and growth in medium containing 1% fetal calf serum. In general, C3H/10T1/2 cells behaved as non-transformed, whether grown at 33 C or 39.5 C. The transformed parental line and all six mutants behave as transformed cells at 33 C. At 39.5 C only the parental transformed line retained the transformed phenotype. Three of the mutants revert towards non-transformed behavior at 39.5 C for all of the properties rested. The remaining mutants are temperature-sensitive for some, but not all, transformed characteristics. Thus, while the expression of these transformed properties is sometimes coupled, the authors have been able to dissociate the expression of traits such as saturation density, anchorage independence and transformed morphology from each other. These mutants should prove to be valuable tools in the study of the mechanisms which underly the expression of the chemically-induced transformed state. |
Supplementary Notes |
Pub. in Carcinogenesis, v1 n12 p1059-1073 1980. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Isolation of Mutants Temperature-Sensitive for Expression of the Transformed State from Chemically Transformed C3H/10Tl/2 Cells. |
PUB Date Free Form |
14 Nov 80 |
Category Codes |
6A; 6C; 57B; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
323801926 |
Origin |
NTIS |
Type |
CAT |