Main Title |
Isolation of a Precursor and a Nascent Chain Form of Glucose-6-Phosphate Dehydrogenase from Rat Uterus and Regulation of Precursor Processing by Estradiol. |
Author |
Cummings, A. M. ;
Barker, K. L. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Texas Tech Univ. Health Sciences Center, Lubbock. |
Year Published |
1986 |
Report Number |
EPA/600/J-86/038; |
Stock Number |
PB86-195484 |
Additional Subjects |
Rats ;
Regulations ;
Isolation ;
Reprints ;
Estradiol ;
Dehydrogenase/glucose-phosphate ;
Precursors ;
Nascent chains
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-195484 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
17p |
Abstract |
SDS-polyacrylamide gel electrophoresis of anti-glucose-6-phosphate dehydrogenase immunoprecipitates from radiolabeled uterine tissue extracts previously revealed three proteins: A, B and C, which were tentatively identified as a 60-64 kDa precursor form, a 57 kDa predominant form, and 40-42 kDa nascent peptide form of the enzyme, respectively. A peptide-mapping technique was used to examine structural homologies among A, B and C. Following the labeling of uterine proteins with (35S)methionine, labeled proteins A, B and C were isolated by immunoprecipitation and electrophoresis. Each protein was individually co-digested with authentic, (3H)methionine-labeled glucose-6-phosphate dehydrogenase using papain, the resulting peptides were resolved by isoelectric focusing and the peptides from the two sources on each gel were compared using double-label counting methods. Proteins A, B and C had at least eight peptides in common, both proteins A and C had two additional peptides in common that were not present in protein B, and B protein had two peptides that were either absent or present in reduced amounts in digests of proteins A and C. The extensive structural homology and immunoreactivity of these proteins indicated that proteins A, B and C were all related to glucose-6-phosphate dehydrogenase. |
Supplementary Notes |
Pub. in Biochimica et Biophysica Acta 880, p226-241 1986. Prepared in cooperation with Texas Tech Univ. Health Sciences Center, Lubbock. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Isolation of a Precursor and a Nascent Chain Form of Glucose-6-Phosphate Dehydrogenase from Rat Uterus and Regulation of Precursor Processing by Estradiol. |
PUB Date Free Form |
c1986 |
Category Codes |
6A; 57B |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
619919481 |
Origin |
NTIS |
Type |
CAT |