| Main Title |
Enzyme-Based Detection of Chlorinated Hydrocarbons. |
| Author |
Offenhartz, B. H. ;
Lefko, J. L. ;
|
| CORP Author |
B and M Technological Services, Inc., Boston, MA.;Municipal Environmental Research Lab., Cincinnati, OH. |
| Year Published |
1984 |
| Report Number |
EPA-68-03-3113; EPA-600/D-84-003; |
| Stock Number |
PB84-125004 |
| Additional Subjects |
Cholinesterase inhibitors ;
Chlorine organic compounds ;
Chemical analysis ;
Environmental surveys ;
Hydrocarbons ;
Catalysis ;
Pesticides ;
Aldrin ;
Immobilized enzymes ;
Toxaphene ;
Aroclor 1242 ;
Phenol/pentachloro
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB84-125004 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
19p |
| Abstract |
Recent advances in immobilized enzyme-based analytical methods, e.g., the cholinesterase-based water monitor 'CAM' (cholinesterase antagonsist monitor), have proved useful in the detection of organophosphate and carbamate pesticides. This work has now been extended to the detection of five high molecular weight chlorinated hydrocarbons at the parts-per-million (ppm) level in a water/methanol matrix. In the new method being developed, the lactate dehydrogenase (LDH) catalyzed reaction: pyruvate + NADH + H(+) yields (LDH) (inhibitor) lactate + NAD(+1) is used to detect the presence of chlorinated hydrocarbons, which reduce the rate of the reaction by inhibiting the enzyme. To date, the method has proved effective in detecting aldrin, toxaphene, Aroclor 1242, DDT, and pentachlorophenol. Nonchlorinated analogs, e.g. phenols, are detected only at orders of magnitude higher concentrations. The method has not yet been evaluated on environmental samples. |
| NTIS Title Notes |
Conference paper Oct 82-Aug 83. |
| PUB Date Free Form |
Jan 84 |
| Category Codes |
7D; 68E#; 99A# |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/14 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
404822443 |
| Origin |
NTIS |
| Type |
CAT |
