| Main Title |
Influence of CC1 (sub 4) Biotransformation on the Activation of Rat Liver Phospholipase C 'In vitro'. |
| Author |
Coleman, J. B. ;
Condie, L. W. ;
Lamb, R. G. ;
|
| CORP Author |
Environmental Protection Agency, Cincinnati, OH. ;Virginia Commonwealth Univ., Richmond. |
| Publisher |
c1988 |
| Year Published |
1988 |
| Report Number |
EPA/600/J-88/314; |
| Stock Number |
PB89-207278 |
| Additional Subjects |
Liver ;
Toxicity ;
In vitro analysis ;
Metabolism ;
Rats ;
Laboratory animals ;
Reprints ;
Carbon tetrachloride ;
Phospholipase C ;
Enzyme activation ;
Biotransformation
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB89-207278 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
Carbon tetrachloride (CCl4) biotransformation and covalent binding was measured in 1000g liver fractions by determining the amount of CCl4 metabolites covalently bound to proteins and lipids at various (5-60 min) incubation times. Reactive intermediate binding to proteins and phospholipids peaked at 20 min, whereas CCl4 metabolites associated with neutral lipids (primarily diacylglycerol) were initially low (0-15 min) and then gradually increased from 20-60 min. The rise in labeled diacylglycerol was associated with a decrease in phospholipids containing covalently bound CCl4 metabolites, since CCl4 bioactivation increased phospholipase C (PLC) activity three- to fourfold. In contrast, when CCl4 bioactivation is absent, 0.5mm CCl4 has little effect on PLC activity. PLC activation by high doses of CCl4 occurs by bioactivation-independent mechanisms. Therefore, there are two components of CCl4-induced PLC activation. Under both conditions, the activation of PLC may be a key event in CCl4 hepatotoxicity since PLC disrupts the functional and structural integrity of membranes by degrading membrane phospholipids. (Copyright (c) 1988 Academic Press, Inc.) |
| Supplementary Notes |
Pub. in Toxicology and Applied Pharmacology, v95 n2 p200-207 Sep 88. See also PB89-207286. Prepared in cooperation with Virginia Commonwealth Univ., Richmond. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Influence of CC1 (sub 4) Biotransformation on the Activation of Rat Liver Phospholipase C 'In vitro'. |
| Category Codes |
57Y; 57B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
925823762 |
| Origin |
NTIS |
| Type |
CAT |
