||In vitro/In vivo Effects of Ethane Dimethanesulfonate on Leydig Cells of Adult Rats.
Klinefelter, G. R. ;
Laskey, J. W. ;
Roberts, N. L. ;
||NSI Technology Services Corp., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
Leydig cells ;
Cyclic adenosine monophosphate ;
Protein kinases ;
In vitro analysis ;
In vivo analysis ;
Electron microscopy ;
Enzyme activation ;
Chorionic gonadotropins ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||Ethane dimethanesulphonate (EDS) is well-recognized as a Leydig cell toxicant. Although this compound has been studied extensively to date, certain toxicological criteria have not been met. For instance, the dose-responsiveness of Leydig cells to EDS, both in vitro and in vivo, is not well established. In addition, the data regarding the cellular site of action of EDS during Leydig cell toxicity and the status of Leydig cell viability during the affected period remains controversial. The study used both highly purified (98 %) and interstitial (14 %) Leydig cell preparations to determine the in vitro EC50 (370 micro M) and in vivio (sub 50) (60 mg/kg) for hCG-stimulated testosterone (T) production, respectively. Leydig cells were recovered in approximately equal number following all in vivo and in vitro EDS exposures. Test results indicate that when Leydig cells are exposed to EDS either in vitro or in vivo, the biosynthesis of T is compromised between the production of cyclic adenosine monophosphate (CAMP) activation of protein kinase and the cholesterol side chain cleavage enzyme.
||Pub. in Toxicology and Applied Pharmacology, v107 n3 p460-471 Mar 91. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
|NTIS Title Notes
||Reprint: In vitro/In vivo Effects of Ethane Dimethanesulfonate on Leydig Cells of Adult Rats.
||PC A03/MF A01