| Main Title |
Characterization of the DNA of a Nonoccluded Baculovirus, Hz-1V. |
| Author |
Huang, Yuan-Shen ;
Hedberg, M. ;
Kawanishi, C. Y. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1982 |
| Report Number |
EPA-600/J-82-113; |
| Stock Number |
PB83-213926 |
| Additional Subjects |
Deoxyribonucleic acids ;
Viruses ;
Cells(Biology) ;
Purification ;
Isolation ;
Electron microscopy ;
Reprints ;
Baculoviruses ;
Cell lines ;
Endonucleases
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB83-213926 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
The DNA of the nonoccluded baculovirus (Hz-1V) obtained from the IMC-Hz-1 cell line was characterized by physicochemical and restriction endonuclease techniques. Hz-1V DNA isolated from purified virus had buoyant densities of 1.58 and 1.54 g/ml in CsC1-ethidium bromide density gradients, which corresponded to supercoiled and to relaxed circular and linear DNA, respectively. Neutral CsC1 equilbrium centrifugation indicated that the Hz-1V DNA had a buoyant density of 1.7024 g/m1, which corresponded to a guanine-plus-cytosine (G + C) content of 43%. Thermal denaturation indicated a high G t C domain (s) in the Hz-1V genomic DNA. The domain (s), which included about 11% of the total genomic DNA, exhibited a Tms corresponded to G t C contents of 42 and 67%, respectively. |
| Supplementary Notes |
Pub. in Jnl. of Virology, p174-181 1982. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Characterization of the DNA of a Nonoccluded Baculovirus, Hz-1V. |
| PUB Date Free Form |
1982 |
| Category Codes |
6M; 57K |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
325919047 |
| Origin |
NTIS |
| Type |
CAT |
