Main Title |
Induction of Activation Antigens on Human Natural Killer Cells Mediated Through the Fc-Gamma Receptor. |
Author |
Harris, D. T. ;
Travis, W. W. ;
Koren., H. S. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Clinical Research Branch. ;Duke Univ. Medical Center, Durham, NC. ;North Carolina Univ. at Chapel Hill. |
Publisher |
c1989 |
Year Published |
1989 |
Report Number |
EPA/600/J-89/467; |
Stock Number |
PB91-116004 |
Additional Subjects |
Antigens ;
Deoxyribonucleic acids ;
Reprints ;
Natural killer cells ;
Fluorescent antibody technic ;
Cross-linking reagents ;
Interleukin-2 ;
Monoclonal antibodies ;
Endogenous substances receptors
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-116004 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
8p |
Abstract |
NK cells, defined here as lymphocytes bearing the CD16 Ag found on the NK cell Fc-gamma receptor (FcR), are known to enter a proliferative and activated state in response to stimulation with IL-2 as assessed by clonal expansion, short-term DNA synthesis, and de novo expression of lymphocyte-associated activation Ag. We have found that the FcR of NK cells acts as a signaling pathway through which IL-2-dependent activation may be greatly enhanced, allowing for more rapid induction of activation Ag and recruitment of an increased percentage of cells expressing surface markers of cellular activation. FcR-interactive agents, such as solid phase immobilized immune complexes or cross-linked CD16-specific mAb, work synergistically with rIL-2 to elicit a rapid expression of IL2R and transferrin receptors on greater than 5% of purified NK cells as early as day 3 of culture. IL-2 or FcR-interactive stimuli alone were weak or ineffective stimulators by comparison. In contrast to the induction of de novo activation Ag, DNA synthesis was elicited by IL-2 alone, but was not substantially or consistently enhanced by the subsequent addition of FcR-interactive stimuli. (Copyright (c) 1989 by the American Association of Immunologists). |
Supplementary Notes |
Pub. in Jnl. of Immunology, v143 n7 p2401-2406 Oct 89. Prepared in cooperation with Duke Univ. Medical Center, Durham, NC., and North Carolina Univ. at Chapel Hill. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Induction of Activation Antigens on Human Natural Killer Cells Mediated Through the Fc-Gamma Receptor. |
Category Codes |
57J; 57B |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
036522940 |
Origin |
NTIS |
Type |
CAT |