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RECORD NUMBER: 332 OF 442Main Title | Review of Methods That Are Used to Determine 'Giardia' Cyst Viability. | |||||||||||
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Author | Schaefer, F. W. ; | |||||||||||
CORP Author | Health Effects Research Lab., Cincinnati, OH. | |||||||||||
Year Published | 1987 | |||||||||||
Report Number | EPA/600/D-87/096; | |||||||||||
Stock Number | PB87-174843 | |||||||||||
Additional Subjects | Giardia ; Cysts ; Viability ; Reviews ; Methodology ; Microscopy ; Fluorescent dyes ; Infectious diseases ; Parasites ; Transmission ; Disinfection ; Excystation | |||||||||||
Holdings |
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Collation | 27p | |||||||||||
Abstract | Over the past 55 years a number of methods have been described for excysting Giardia cysts as a means of determining viability. The excystation methods for G. muris cysts are reliable and reproducible. However, methods published to date for the excystation of G. lamblia cysts, the human pathogen, have not yielded reliable, reproducible results. For both Giardia cyst types the stimulatory factors promoting excystation are low pH, carbon dioxide, a temperature around 37 C, and a final neutralizing step at pH 7.0. Recently, other methods have been developed for determining Giardia cyst viability. These include several fluorescent vital dyes, differential interference contrast microscopy, and Mongolian gerbil infectivity tests. | |||||||||||
PUB Date Free Form | Mar 87 | |||||||||||
Category Codes | 57N; 57K; 57U | |||||||||||
NTIS Prices | PC A03/MF A01 | |||||||||||
Primary Description | 600/11 | |||||||||||
Document Type | NT | |||||||||||
Cataloging Source | NTIS/MT | |||||||||||
Control Number | 718319708 | |||||||||||
Origin | NTIS | |||||||||||
Type | CAT |