| Main Title |
Differential Effect of Tetrazolium Dyes upon Bacteriophage Plaque Assay Titers. |
| Author |
Hurst, C. J. ;
Blannon, J. C. ;
Hardaway, R. L. ;
Jackson, W. C. ;
|
| CORP Author |
Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Environmental Careers Organization, Tampa, FL. |
| Publisher |
cSep 94 |
| Year Published |
1994 |
| Report Number |
EPA/600/J-94/428; |
| Stock Number |
PB95-122628 |
| Additional Subjects |
Bioassay ;
Dyes ;
Bacteriophages ;
Water pollution ;
Tetrazolium ;
Microbiology ;
Plaque formation ;
Bacteriology ;
Viruses ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB95-122628 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
The study examined whether the practice of incorporating either tetrazolium red or tetrazolium violet dye into plaque assay medium deleteriously influences plaque assay titers. Representative members of six different virus families were studied: Cystoviridae (Phi6), Leviviridae (MS2), Microviridae (PhiX174), Myoviridae (T2), Podoviridae (P22), and Siphoviridae (Denver, T1, and VD13). Each of the members of the Podoviridae and Siphoviridae families appeared to be suppressed by either one or both dyes at a 300-micrograms/ml concentration. The chosen representatives of the other bacteriophage families were not suppressed by either dye at a 300-micrograms/ml concentration. Subsequent trials revealed no suppression of Podoviridae or Siphoviridae plaque assay titers when members of these virus families were tested with the same two dyes at the lower concentrations of 150 and 50 micrograms/ml. Interestingly, the bacteriohage families whose members were affected by the dyes have additional commonality in that they are the two bacteriophage families whose members were affected by the dyes have additional commonality in that they are the two bacteriophage families whose members possess both double-stranded DNA genomes and noncontractile tails. (Copyright (c) 1994, American Society for Microbiology.) |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v60 n9 p3462-3465 Sep 94. Prepared in cooperation with Environmental Careers Organization, Tampa, FL. |
| NTIS Title Notes |
Journal article 1993-94. |
| Title Annotations |
Reprint: Differential Effect of Tetrazolium Dyes upon Bacteriophage Plaque Assay Titers. |
| Category Codes |
57K; 68D |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/14 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
433518311 |
| Origin |
NTIS |
| Type |
CAT |
