| Main Title |
Direct Measurement of Fast Axonal Organelle Transport in the Sciatic Nerve of Rats Treated with Acrylamide. |
| Author |
Padilla, S. ;
Atkinson, M. B. ;
Breuer, A. C. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Cleveland Clinic Foundation, OH. |
| Publisher |
c1993 |
| Year Published |
1993 |
| Report Number |
EPA/600/J-94/388; |
| Stock Number |
PB95-126553 |
| Additional Subjects |
Axons ;
Organelles ;
Sciatic nerve ;
Acrylamides ;
Toxicology ;
Rats ;
Dose-response relationships ;
Microscopy ;
Body weight ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB95-126553 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
20p |
| Abstract |
The effects of acrylamide on fast axonal transport have been measured primarily using the indirect methods of isotope or enzyme accumulation. The authors report the first direct evaluation of the effects of subchronic acrylamide dosing (150, 300, or 500 mg/kg total dose, i.e., 50 mg/kg, 2x/wk, for 1.5, 3, 5 wk, respectively) on the fast axonal transport motility machinery itself using video-enhanced differential interference contrast optics with digital image processing and computer analysis. Four principle observations were made: (1) Rapid anterograde transport was not affected at any dosage level within 1 wk after cessation of dosing. (2) A high cumulative dosage (500 mg/kg total) of acrylamide or bisacrylamide produced approximately 7-18% decrease in the rate of retrograde transport in both myelinated and unmyelinated axons. (3) Lower dosages of acrylamide (150 or 300 mg/kg total) produced an increase in retrograde transport rates in myelinated axons only. (4) During the recovery phase for the 500 mg/kg acrylamide animals (i.e., 3 or 5 wk after the last dosage of acrylamide) the rate of anterograde transport in the myelinated axons was decreased at 3 wk but not at 5 wk, and the rate of retrograde transport in the mylinated axons returned to control levels while the retrograde transport in the unmyelinated axons continued at abnormally slow speeds. |
| Supplementary Notes |
Pub. in Jnl. of Toxicology and Environmental Health 39, n4 p429-445 Aug 93. Prepared in cooperation with Cleveland Clinic Foundation, OH. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Direct Measurement of Fast Axonal Organelle Transport in the Sciatic Nerve of Rats Treated with Acrylamide. |
| Category Codes |
57Y; 57I |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
435318915 |
| Origin |
NTIS |
| Type |
CAT |
