| Main Title |
Embryonic Palatal Responses to Teratogens in Serum-Free Organ Culture. |
| Author |
Abbott, B. D. ;
Buckalew, A. R. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA/600/J-92/190; |
| Stock Number |
PB92-188960 |
| Additional Subjects |
Teratogenic compounds ;
Organ culture ;
Palate ;
Embryo ;
Serum-free media ;
Dose-response relationships ;
In vivo analysis ;
In vitro analysis ;
Tretinoin ;
Triamcinolone ;
Tetrachlorodibenzodioxins ;
Hydrocortisone ;
Dexamethasone ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-188960 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
17p |
| Abstract |
The study examines development of rat, mouse, and human embryonic palates in submerged, serum-free organ culture. The concentration-response profiles for retinoic acid (RA), triamcinolone (TRI), hydrocortisone (HC), dexamethasone (DEX), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were examined and the mechanisms of clefting in vitro were compared to observed in vivo responses. The present study demonstrates that serum-free organ culture supports development of mouse, rat, and human palatal explants. The present study also demonstrates the capacity of this organ culture system to model palatogenesis for several species, and to distinguish between various mechanisms of clefting as presented through selected model compounds. This model should be useful for exploring mechanisms of activity at a cellular and molecular level. |
| Supplementary Notes |
Pub. in Teratology, v45 n4 p369-382 Apr 92. Prepared in cooperation with ManTech Environmental Technology, Inc., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Embryonic Palatal Responses to Teratogens in Serum-Free Organ Culture. |
| Category Codes |
57Y |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
220521512 |
| Origin |
NTIS |
| Type |
CAT |
