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OLS Field Name OLS Field Data
Main Title Role of Glutathione Depletion in the Cytotoxicity of Acetaminophen in a Primary Culture System of Rat Hepatocytes.
Author Mitchell, D. B. ; Acosta, D. ; Bruckner, J. V. ;
CORP Author Procter and Gamble Co., Cincinnati, OH. Miami Valley Labs. ;Texas Univ. at Austin. Dept. of Pharmacology and Toxicology. ;Georgia Univ., Athens. Dept. of Pharmacology and Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/343;
Stock Number PB86-175387
Additional Subjects Toxicology ; Rats ; Hepatocytes ; Depletion ; Laboratory animals ; Reprints ; Cytotoxicity ; Acetaminophen ; Glutathione
Holdings
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NTIS  PB86-175387 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 22p
Abstract A primary culture system of postnatal rat hepatocytes was utilized to study the cytotoxicity of acetaminophen and the toxicological significance of glutathione (GSH) depletion. The relative time of onset and magnitude of GSH depletion, lipid peroxidation and cytotoxicity were contrasted in order to gain insight into their interrelationships. Exposure of the hepatocytes to acetaminophen resulted in time-and dose-dependent depletion of cellular GSH. The acetaminophen-induced GSH depletion and ensuing lactate dehydrogenase (LDH) leakage were quite modest and delayed in onset, in contrast to that caused by iodoacetamide (IAA) and by diethylmaleate (DEM), 2 well-known depletors of GSH. There was comparable LDH leakage, irrespective of drug treatment, when GSH levels decreased to about 20% of normal. Reduction of GSH levels below the 20% threshold by IAA treatment resulted in marked LDH leakage and loss of viability.
Supplementary Notes Pub. in Toxicology 37, p127-146 Oct 85. Prepared in cooperation with Texas Univ. at Austin. Dept. of Pharmacology and Toxicology, and Georgia Univ., Athens. Dept. of Pharmacology and Toxicology. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Role of Glutathione Depletion in the Cytotoxicity of Acetaminophen in a Primary Culture System of Rat Hepatocytes.
PUB Date Free Form c1985
Category Codes 6T; 57Y
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 614222661
Origin NTIS
Type CAT