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Main Title Analytical Procedures and Quality Assurance Criteria for the Determination of Major and Minor Deoxynucleosides in Fish Tissue DNA by Liquid Chromatography-Ultraviolet Spectroscopy and Liquid Chromatography Thermospray Mass Spectrometry.
Author Serrano, J. ; Kuehl, D. W. ; Naumann, S. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;AScI Corp., Duluth, MN.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/277;
Stock Number PB93-222354
Additional Subjects Chemical analysis ; Deoxyribonucleic acids ; Nucleosides ; Fishes ; Liquid chromatography ; Ultraviolet spectrophotometry ; Mass spectroscopy ; Quality control ; Reprints ;
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NTIS  PB93-222354 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Abstract Analytical procedures and quality assurance criteria have been established for enzymatic hydrolysis of fish tissue DNA to free nucleosides and their subsequent characterization by liquid chromatography-photodiode-array ultraviolet spectroscopy and liquid chromatography-thermospray mass spectrometry. Optimization of enzymatic efficiency to assure minimal loss of modified nucleosides is described. Variability in analyte capacity factors and multiwavelength response have been compared for analyte standards and hydrolysates, and results have been used to derive qualitative and quantitative quality assurance criteria. A comparison of DNA mole percent calculated using single-wavelength quantification and multiwavelength averaging quantification indicates that less variability in data may be expected using the multiwavelength technique. Finally, the comparison of DNA from liver of three species of fish widely used in mutagen/carcinogen laboratory and field studies, rainbow trout (Onchorynchus mykiss), medaka (Oryzias latipes), and brown bullhead (Ictalurus nebulosus), has been determined. Identification of deoxyuridine in the DNA hydrolysates of each fish indicates that this analyte should be measured to accurately report DNA deoxynucleoside mole percent, especially when reporting data for the methylation of deoxycytidine. (Copyright (c) 1993 Elsevier Science Publishers B.V.)
Supplementary Notes Pub. in Jnl. of Chromatography 615, p203-213 1993. Prepared in cooperation with AScI Corp., Duluth, MN.
NTIS Title Notes Journal article.
Title Annotations Reprint: Analytical Procedures and Quality Assurance Criteria for the Determination of Major and Minor Deoxynucleosides in Fish Tissue DNA by Liquid Chromatography-Ultraviolet Spectroscopy and Liquid Chromatography Thermospray Mass Spectrometry.
Category Codes 57F; 99A
NTIS Prices PC A03/MF A01
Primary Description 600/03
Document Type NT
Cataloging Source NTIS/MT
Control Number 326626566
Origin NTIS
Type CAT