| Main Title |
Breakage and Binding of DNA by Reaction Products of Hypochlorous Acid with Aniline, 1-Napthylamine, or 1-Naphthol. |
| Author |
Kozumbo, W. J. ;
Agarwal, S. ;
Koren, H. S. ;
|
| CORP Author |
North Carolina Univ. at Chapel Hill. Center for Environmental Medicine and Lung Biology.;Health Effects Research Lab., Research Triangle Park, NC.;National Institutes of Health, Bethesda, MD. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA/600/J-93/118; |
| Stock Number |
PB93-181063 |
| Additional Subjects |
DNA damage ;
Deoxyribonucleic acids ;
Aniline ;
1-napthylamine ;
Naphthols ;
Hypochlorous acids ;
Binding sites ;
Toxicology ;
Neutrophils ;
In vitro analysis ;
Ultraviolet spectrophotometry ;
Antioxidants ;
Fibroblasts ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-181063 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
Inhaled tobacco smoke and urban smog can elicit inflammatory neutrophils into the lung. They also contain aryl compounds absorbed to particles which, when phagocytized by neutrophils or monocytes trigger intraphagosomal and extracellular release of hypochlorous acid (HOC1), a potently reactive oxidant. Using aniline, 1-naphthylamine and 1-napthol (1-NOH) as model pollutant compounds, we examined whether HOC1 (OC1) could potentially transform phagocytized compounds into genotoxic products. Compounds (15-25 micro M) were first reacted with HOC1 (25-150 micro M) in phosphate buffer and then used to treat diploid human lung fibroblasts or purified DNA. DNA single-strand breaks/alkali-labile sites were assayed in cells by DNA alkaline elution, and binding of HOC1-reacted ((14)C) 1NOH to purified DNA was analyzed by scintillation spectrometry. Neither HOC1 nor compounds alone could break DNA, but HOC1-reacted compounds induced up to 400 red equivalents of DNA damage. |
| Supplementary Notes |
Pub. in Toxicology and Applied Pharmacology 115, n1 p107-115 Jul 92. Sponsored by Health Effects Research Lab., Research Triangle Park, NC., and National Institutes of Health, Bethesda, MD. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Breakage and Binding of DNA by Reaction Products of Hypochlorous Acid with Aniline, 1-Napthylamine, or 1-Naphthol. |
| Category Codes |
57Y; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
316134232 |
| Origin |
NTIS |
| Type |
CAT |
