| Main Title |
Effects of Betaine on Enumeration of Airborne Bacteria. |
| Author |
Marthi, B. ;
Lighthart., B. ;
|
| CORP Author |
Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA/600/J-90/197; |
| Stock Number |
PB91-116210 |
| Additional Subjects |
Pseudomonas ;
Bacteria ;
Aerosols ;
Sewage treatment ;
Airborne ;
Concentration(Composition) ;
Monitoring ;
Counting ;
Fluids ;
Growth ;
Prevention ;
Disease vectors ;
Osmosis ;
Stress(Physiology) ;
Dehydration ;
Enzymes ;
Stabilization ;
Reprints ;
Betaine ;
Osmoprotectant ;
Genetic engineering ;
Sewage treatment plants
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-116210 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
The osmoprotectant betaine was incorporated into collection fluid and enumeration medium to determine its effects on the colony-forming abilities of airborne bacteria, which were collected from three separate locations: a wastewater treatment plant, the roof of a laboratory building, and an unobstructed farmland. At all locations, addition of 2 to 5 mM betaine caused a significant increase (from 21.6 to 61.3%) in colonial outgrowth, compared with the growth rate of controls without betaine. The presence of betaine in both the collection fluid and the enumeration medium had an additive effect on the colony-forming ability of airborne bacteria compared with the presence of betaine in either one alone. The effect of various betaine concentrations on the enumeration of aerosolized Pseudomonas syringae was determined. Betaine showed a threshold for maximum effect at a concentration of 2 to 5 mM. The significance of these results with respect to the development of protocols for monitoring airborne microorganisms, including genetically engineered microorganisms, is discussed. |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v56 n5 p1286-1289 Nov 89. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Effects of Betaine on Enumeration of Airborne Bacteria. |
| Category Codes |
57K#; 57E; 57W |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/02 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
036523084 |
| Origin |
NTIS |
| Type |
CAT |
