| Main Title |
In vitro Metabolism and DNA Adduct Formation from the Mutagenic Environmental Contaminant 2-Nitrofluoranthene. |
| Author |
Herreno-Saenz, D. ;
Evans, F. E. ;
Heinze, T. ;
Lewtas, J. ;
Fu, P. P. ;
|
| CORP Author |
National Center for Toxicological Research, Jefferson, AR.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA/600/J-93/340; |
| Stock Number |
PB93-229581 |
| Additional Subjects |
Metabolic activation ;
DNA damage ;
Mutagens ;
Environmental pollutants ;
Liquid chromatography ;
Rats ;
In vitro analysis ;
Liver microsomes ;
Nuclear magnetic resonance ;
Enzyme inhibitors ;
Epoxide hydrolases ;
Carcinogens ;
Mass spectroscopy ;
pH ;
Reprints ;
2-Nitrofluoranthene
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-229581 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
The metabolism and DNA adduct formation by the mutagenic environmental contaminant 2-nitrofluoranthene (2-NFA) was studied. Incubation under aerobic conditions with liver microsomes of rats pretreated with 3-methylcholanthrene yielded trans-7,8-dihydroxy-7,8-dihydro-2-nitrofluoranthene, trans-9,10-dihydroxy-9,10-dihydro-2-nitrofluoranthene, and 7-, 8-, and 9-phenolic metabolites. When the epoxide hydrolase inhibitor, 3,3,3-trichloropropylene was present in the incubation, only phenolic metabolites were detected. Under hypoxic conditions, 2-aminofluoranthene was obtained, together with a trace of the ring-oxidized metabolites. The activated metabolite, N-hydroxy-2-aminofluoranthene, was prepared in situ and reacted with calf thymus DNA. Upon enzymatic hydrolysis of the DNA and purification by HPLC, a C8-substituted deoxyguanosine adduct, N-(deoxyguanosin-8-yl)-2-aminofluoranthene, was identified by mass and proton NMR spectral analysis. This adduct was also formed at a level of 10 pmol/mg of DNA when 2-NFA was metabolized by xanthine oxidase, 6 pmol/mg of DNA from incubation with liver microsomes of rats pretreated with 3-methylcholanthrene, and 3 pmol/mg of DNA from metabolism by liver microsomes of rats pretreated with phenobarbital. |
| Supplementary Notes |
Pub. in Toxicology, v5 n6 p863-869 Nov/Dec 92. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: In vitro Metabolism and DNA Adduct Formation from the Mutagenic Environmental Contaminant 2-Nitrofluoranthene. |
| Category Codes |
57Y; 57B; 57F; 68G |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
328023552 |
| Origin |
NTIS |
| Type |
CAT |
