| Main Title |
Isolation and Long-Term Culture of Rat, Rabbit, and Human Nasal Turbinate Epithelial Cells. |
| Author |
Steele, V. E. ;
Arnold, J. T. ;
|
| CORP Author |
Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1986 |
| Report Number |
EPA-68-02-4032; EPA/600/J-85/446; |
| Stock Number |
PB86-239902 |
| Additional Subjects |
Cells(Biology) ;
Rats ;
Rabbits ;
Humans ;
Isolation ;
Cultures(Biology) ;
Reprints ;
Epithelial cells
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-239902 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
Nasal turbinate epithelial cells were isolated from rats, rabbits, and humans using either a surgical or an in situ enzyme incubation technique. The culture conditions that permit optimal cell attachment and selective growth of the nasal epithelial cells were determined. These conditions will permit the long-term culture of these cells where typically 20 to 30 population doublings were observed. Differences between rat and human nasal epithelial cells were seen in substrate requirements, colony-forming efficiency, and response to fetal bovine serum and bovine serum albumin. These methodology and results will permit mechanistic studies of normal and abnormal cellular function and permit comparative response studies between nasal epithelial cells from rats and humans. |
| Supplementary Notes |
Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| Availability Notes |
Pub. in Vitro Cellular and Developmental Biology, v21 n12 p681-687, Dec 85. |
| Title Annotations |
Reprint: Isolation and Long-Term Culture of Rat, Rabbit, and Human Nasal Turbinate Epithelial Cells, |
| PUB Date Free Form |
c1986 |
| Category Codes |
6C; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
631013944 |
| Origin |
NTIS |
| Type |
CAT |
