| Main Title |
Ammonia Removal from Mammalian Cell Culture Medium by Ion-Exchange Membranes. |
| Author |
Sikdar, S. K. ;
Sawant, S. B. ;
|
| CORP Author |
Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Bombay Univ. (India). Dept. of Chemical Technology. |
| Publisher |
c1994 |
| Year Published |
1994 |
| Report Number |
EPA/600/J-94/371; |
| Stock Number |
PB95-124863 |
| Additional Subjects |
Ammonia ;
Cultured cells ;
Culture media ;
Ion exchange resins ;
Artifical membrane ;
Lactic acid ;
Reprints ;
Perfluorosulfonic acid polymer membranes
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB95-124863 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
13p |
| Abstract |
Metabolites such as ammonia and lactic acid formed during mammalian cell culture can frequently be toxic to the cells themselves beyond a threshold concentration of the metabolites. Cell culture conducted in the presence of such accumulated metabolites is therefore limited in productivity. This work demonstrates with laboratory data that a nonporous ion-exchange membrane of the perfluorinated sulfonic acid type can be used to contact the culture medium, and ammonia removed selectivity from the medium without disturbing the process. The technique of pervaporation showed particular promise in this regard. The pervaporation used with inert gas sweep on the permeate side was found superior to that used with vacuum application. |
| Supplementary Notes |
Pub. in Separation Science and Technology 29, n12 p1579-1591 1994. Prepared in cooperation with Bombay Univ. (India). Dept. of Chemical Technology. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Ammonia Removal from Mammalian Cell Culture Medium by Ion-Exchange Membranes. |
| Category Codes |
57F; 99F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/14 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
435317630 |
| Origin |
NTIS |
| Type |
CAT |
