| Main Title |
Anomalies in the Enumeration of Starved Bacteria on Culture Media Containing Nalidixic Acid and Tetracycline. |
| Author |
Genthner, F. J. ;
Upadhyay, J. ;
Campbell, R. P. ;
Genthner, B. R. S. ;
|
| CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Virginia Polytechnic Inst. and State Univ., Blacksburg. ;Technical Resources, Inc., Gulf Breeze, FL. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA/600/J-90/515; |
| Stock Number |
PB91-206870 |
| Additional Subjects |
Pseudomonas fluorescens ;
Culture media ;
Tetracycline ;
Nalidixic acid ;
Antibiotic resistance ;
Microbial drug resistance ;
Genetic engineering ;
Catalase ;
Starvation ;
Microbial colony count ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-206870 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
Culturable counts of antibiotic resistant, genetically engineered Pseudomonas fluorescens were determined on antibiotic-containing plate count agar during starvation in water. The mean of the colony counts on plate count agar with or without tetracycline (4.9 times ten to the sixth power/ml) was significantly higher than mean colony counts on plate count agar containing either nalidixic acid or nalidixic acid plus tetracycline (2.5 times ten to the sixth power/ml). After 20 days of starvation the highest mean colony counts continued to be obtained on plate count agar (7.2 times ten to the sixth power/ml) with slightly, but significantly, lower counts obtained on plate count agar containing either nalidixic acid (5.6 times ten to the sixth power/ml) or tetracylcline (1.5 times ten to the sixth power/ml). A combination of nalidixic acid and tetracycline in plate count agar, however, dramatically reduced colony counts (8.3 x 100/ml) after the starvation period. The addition of catalase to plate count agar containing nalidixic acid and tetracycline negated the effect caused by the combination. |
| Supplementary Notes |
Pub. in Microbial Ecology 20, p283-288 1990. Prepared in cooperation with Virginia Polytechnic Inst. and State Univ., Blacksburg, and Technical Resources, Inc., Gulf Breeze, FL. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Anomalies in the Enumeration of Starved Bacteria on Culture Media Containing Nalidixic Acid and Tetracycline. |
| Category Codes |
57K; 57Q; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
124123180 |
| Origin |
NTIS |
| Type |
CAT |
