| Main Title |
'Giardia muris': Scanning Electron Microscopy of In vitro Excystation. |
| Author |
Coggins, J. R. ;
Schaefer,III, F. W. ;
|
| CORP Author |
Health Effects Research Lab., Cincinnati, OH. ;Wisconsin Univ.-Milwaukee. Dept. of Zoology. |
| Year Published |
1984 |
| Report Number |
EPA/600/J-84-081; |
| Stock Number |
PB84-244094 |
| Additional Subjects |
Electron microscopy ;
Parasites ;
In vitro analysis ;
Mice ;
Cultivation ;
Reprints ;
Giardia muris ;
Excystation
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB84-244094 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
A recently developed in vitro excystation procedure results in almost total excystation of Giardia muris, an intestinal parasite of mice. The present experiment examines the G. muris cyst morphology by scanning electron microscopy and evaluates the efficacy of the excystation procedure. Untreated cysts of G. muris were elliptical and displayed a distinctive surface structure. Excystation began almost immediately after incubation had begun and most trophozoites emerged within 30 minutes. Excystation appears to involve flagellar action of the encysted trophozoite. A tear of the wall occurred at one pole. This opening was subsequently enlarged, presumably by flagellar action. Trophozoites emerged, posterior end first, and an associated mucoid-like material was extruded. Newly emerged trophozoites were nearly oval in shape. Trophozoites quickly became flattened, elongated and underwent cytokinesis resulting in two daughter trophozoites. Few non-excysted organisms were seen after 30 minutes incubation. |
| Supplementary Notes |
Prepared in cooperation with Wisconsin Univ.-Milwaukee. Dept. of Zoology. |
| Availability Notes |
Pub. in Experimental Parasitology 57, p62-67 1984. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: 'Giardia muris': Scanning Electron Microscopy of In vitro Excystation. |
| PUB Date Free Form |
c1984 |
| Category Codes |
6C; 57N |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
435625593 |
| Origin |
NTIS |
| Type |
CAT |
