| Main Title |
Interactive Regulation of Ah and Glucocorticoid Receptors in the Synergistic Induction of Cleft Palate by 2,3,7,8-Tetrachlorodibenzo-p-dioxin and Hydrocortisone. |
| Author |
Abbott, B. D. ;
Perdew, G. H. ;
Buckalew, A. R. ;
Birnbaum, L. S. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Perinatal Toxicology Branch. ;Purdue Univ., Lafayette, IN. Dept. of Foods and Nutrition. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC. |
| Publisher |
c1994 |
| Year Published |
1994 |
| Report Number |
EPA/600/J-94/550; |
| Stock Number |
PB95-148110 |
| Additional Subjects |
Glucocorticoid receptors ;
Aryl hydrocarbon receptors ;
Teratogenic compounds ;
Cleft palate ;
Hydrocortisone ;
Mice ;
Hydronephrosis ;
Messenger RNA ;
Immunohistochemistry ;
In situ hybridization ;
Northern blotting ;
Dose-response relationships ;
Down-regulation(Physiology) ;
Reprints ;
Tetrachlorodibenzodioxins
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB95-148110 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
15p |
| Abstract |
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a widespread environmental contaminant the produces adverse biological effects including carcinogenesis, reproductive toxicity, immune dysfunction, hyperderatosis, hepatotoxicity, thymic involution, and teratogenesis. The present study examines the expression of Ah receptor (AhR) and glucocorticoid receptor (GR) in the embryonic palate following exposure to TCDD, hydrocortison (HC), and HC + TCDD. C57BL/6N pregnant mice were treated with HC (25 or 100 mg/kg/day GD10-13, sc), TCDD (3 microgram/kg/day GD10-13, or 23 microgram/kg GD10, orally), or HC + TCDD (25 mg/kg/day SC and 3 microgram/kg/day orally, GD10-13). Craniofacial tissues were collected from the embryos on GD14 and examined for AhR and GR expression using in situ hybridization, Northern blots, and immunohistochemistry. The authors found that in the embryonic palate exposed to TCDD, the AhR was downregulated and the GR expression increased. Conversely, following HC exposure, the GR was downregulated and AhR levels were elevated. HC + TCDD produced increased expression of both receptors. Effects on AhR appeared to be regulated at the transcriptional level, as both protein and mRNA were altered in similar directions. |
| Supplementary Notes |
Pub. in Toxicology and Applied Pharmacology 128, n1 p138-150 Sep 94. Prepared in cooperation with Purdue Univ., Lafayette, IN. Dept. of Foods and Nutrition. and ManTech Environmental Technology, Inc., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Interactive Regulation of Ah and Glucocorticoid Receptors in the Synergistic Induction of Cleft Palate by 2,3,7,8-Tetrachlorodibenzo-p-dioxin and Hydrocortisone. |
| Category Codes |
57Y; 57B; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
503414735 |
| Origin |
NTIS |
| Type |
CAT |
