| Main Title |
Comparison of Mutagenicity Results for Nine Compounds Evaluated at the 'hgprt' Locus in the Standard and Suspension CHO Assays. |
| Author |
Moore, M. M. ;
Parker, L. ;
Huston, J. ;
Harrington-Brock, K. ;
Dearfield, K. L. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-91/019; |
| Stock Number |
PB91-183475 |
| Additional Subjects |
Mutagens ;
Chromosome mapping ;
Hypoxanthine phosphoribosyltransferase ;
Mutagenicity tests ;
Chinese hamsters ;
Comparative evaluations ;
Acrylates ;
Ethyl methanesulfonate ;
Methyl methanesulfonate ;
Acridines ;
Tables(Data) ;
Dose-response relationships ;
Thymidine kinase ;
Reprints ;
Mouse lymphoma assay
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-183475 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
The Chinese hamster ovary (CHO) assay, which measures newly induced mutations at the hypoxanthine-guanine phosphoribosyltransferase (hgprt) locus, has been widely used for mutagenesis testing. The insensitivity of the standard assay to some genotoxic agents has been speculated to be due to the relatively small number of cells used in the assay. The present study compares the standard monolayer assay with a suspension adapted assay which uses cell numbers comparable to that of the L5178Y mouse lymphoma assay. Nine compounds, ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), 2-methoxy-6-chloro-9-(3-(ethyl-2-chloroethyl)-aminopropylamino)-acridine 2HCl (ICR 170), methyl acrylate, ethyl acrylate, tetraethylene glycol diacrylate, trimethylolpropane triacrylate, 2-ethylhexyl acrylate, and dicyclopentenyloxyethyl methacrylate were evaluated in the monolayer and suspension assays. Both assays gave the same overall positive/negative evaluation for the test compounds. There were some quantitative differences in the mutant frequency for the three compounds found to be mutagenic (EMS, MMS, and ICR 170). The acrylates (many of which appear to exert their genotoxic effect through a clastogenic mechanism) were negative in both test systems. The use of the suspension assay did not improve the ability of the hgprt locus to detect the genotoxicity of the acrylates. |
| Supplementary Notes |
Pub. in Mutagenesis, v6 n1 p77-85 Jan 91. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Comparison of Mutagenicity Results for Nine Compounds Evaluated at the 'hgprt' Locus in the Standard and Suspension CHO Assays. |
| Category Codes |
57F; 57Y |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
117927388 |
| Origin |
NTIS |
| Type |
CAT |
