||Catabolism of Aromatic Biogenic Amines by 'Pseudomonas aeruginosa' PA01 via meta Cleavage of Homoprotocatechuic Acid (Journal Version).
Cuskey, S. M. ;
Olsen, R. H. ;
||Environmental Research Lab., Gulf Breeze, FL. ;Michigan Univ., Ann Arbor. Medical School.
Biogenic amines ;
Pseudomonas aruginosa ;
NADPH oxidoreductases ;
Enzyme induction ;
Molecular cloning ;
Chromosome mapping ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||Pseudomonas aruginosa PA01 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). Meta ring cleavage was mediated by 3-4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-dependent dehydrogenase caused the disappearance of the yellow MSA product, probably forming 2-hydroxy-5-carboxymethylmuconic acid. Induction studies with extracts from mutant cells indicated that the inducer of HPADO was HPA and/or MSA. Strains PA01.221 (tynCl) and PA01.303 (tynDI) have chromosomal mutations causing a deficiency in the activity necessary for conversion of 4-hydroxyphenylacetic acid to HPA. (Copyright (c) 1988 American Society for Microbiology.)
||Prepared in cooperation with Michigan Univ., Ann Arbor. Medical School.
||Pub. in Jnl. of Bacteriology, v170 n1 p393-399 Jan 88.
|NTIS Title Notes
||Reprint: Catabolism of Aromatic Biogenic Amines by 'Pseudomonas aeruginosa' PA01 via meta Cleavage of Homoprotocatechuic Acid (Journal Version).
||57K; 57B; 57F
||Not available NTIS