| Main Title |
Using Neuroblastoma Cell Lines to Examine Organophosphate Neurotoxicity. |
| Author |
Veronesi, B. ;
Ehrich, M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Virginia-Maryland Regional Coll. of Veterinary Medicine, Blacksburg, VA. |
| Publisher |
c1993 |
| Year Published |
1993 |
| Report Number |
EPA/600/J-93/332; |
| Stock Number |
PB93-229508 |
| Additional Subjects |
Toxicity ;
Nervous system ;
Neuroblastoma ;
Organophosphate insecticides ;
Cultured tumor cells ;
In vitro analysis ;
Esterases ;
Cholinesterase inhibitors ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-229508 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
The paper describes the initial characterization of neuroblastoma cell lines to address several aspects of organophosphate neurotoxicity. Several commercially available human and mouse cell lines (i.e., SY5Y, IMR-32, SK-N-MC, NB41A3) were evaluated for their target esterase activities (i.e., cholinesterase, neurotoxic esterase, carboxylesterase), and of these cells, a human (SY5Y) and mouse (NB41A3) neuroblastoma cell line clone were used to establish an IC50 cytoxicity profile for a variety of organophosphates insecticides (e.g., parathion, paraoxon, diisopropylphosphorofluoridate and mipafox). The human neuroblastoma cell line clone (SY5Y) was further used to distinguish between neuropathy-causing OPs and cholinesterase inhibitors. These initial data support the use of neuroblastoma cell lines as effective test models for organophosphate neurotoxicity. |
| Supplementary Notes |
Pub. in In vitro Toxicology: A Jnl. of Molecular and Cellular Toxicology, v6 n1 p57-65 1993. Prepared in cooperation with Virginia-Maryland Regional Coll. of Veterinary Medicine, Blacksburg, VA. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Using Neuroblastoma Cell Lines to Examine Organophosphate Neurotoxicity. |
| Category Codes |
57Y; 57F; 68E |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
328023507 |
| Origin |
NTIS |
| Type |
CAT |
