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OLS Field Name OLS Field Data
Main Title Improved Method for the Determination of Aryl-4-hydroxylase Activity.
Author Lindblad, William J. ; Jackim, Eugene ;
CORP Author Environmental Research Lab., Narragansett, RI.
Year Published 1981
Report Number EPA-600/J-81-525 ;ERLN-J185;
Stock Number PB82-167230
Additional Subjects Filtration ; Separation ; Enzymes ; Revisions ; Toxicology ; Extraction ; Fluorescence ; Cellulose acetate ; Reprints ; Hydroxylases/aryl ; Tritium release assay ; Benzopyrenes
Library Call Number Additional Info Location Last
NTIS  PB82-167230 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 3p
Abstract An improved method for separation of tritiated benzo(a) pyrene from tritiated water in the aryl-4-hydroxylase assay is presented. Quantitative retention of benzo(a) pyrene was obtained with mixed cellulose acetate and nitrate filters. Enzymatic activities obtained with this modification agreed well with those determined using the standard charcoal column extraction. This modification greatly reduces the time required to perform this assay, and allows for the determination of enzyme activity in a large number of samples.
Supplementary Notes Pub. in Jnl. of Applied Toxicology, v1 n4 p234-235 1981.
NTIS Title Notes Journal article.
Title Annotations Reprint: An Improved Method for the Determination of Aryl-4-hydroxylase Activity.
PUB Date Free Form 1981
Category Codes 7D; 99
NTIS Prices PC A02/MF A01
Primary Description 600/05
Document Type NT
Cataloging Source NTIS/MT
Control Number 323811504
Origin NTIS
Type CAT