| Main Title |
Metal-Induced Alteration of the Cell Membrane/Cytoplasm Complex Studied by Flow Cytometry and Detergent Lysis. |
| Author |
Zucker, R. M. ;
Elstein, K. H. ;
Easterling, R. E. ;
Massaro, E. J. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;NSI Technology Services Corp., Research Triangle Park, NC. |
| Publisher |
c1988 |
| Year Published |
1988 |
| Report Number |
EPA/600/J-88/487; |
| Stock Number |
PB90-185190 |
| Additional Subjects |
Toxicology ;
Cadmium ;
Zinc ;
Cytoplasm ;
Detergents ;
Fluorescence ;
Cell nucleus ;
Reprints ;
Tributyltin ;
Cell membrane ;
Cultured tumor cells ;
Flow cytometry
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-185190 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
Flow cytometric analysis of the cell cycle is most effectively accomplished with membrane-/cytoplasma-free (clean) nuclei. Non-ionic detergents (e.g. NP40 or Triton X-100) commonly are employed to solubilize cells membranes/cytoplasm to produce 'clean' nuclei. Treatment of murine erythroleukemic cells with tri-n-butylin methoxide, cadmium acetate, zinc sulfate, or lead acetate alters the properties of the cell membrane/cytoplasm complex making it resistant to NP40 dissolution. On a molar basis, the organotin compound was more effective in inducing resistance to detergent-mediated dissolution than the inorganic metal compounds. Resistance to NO40-mediated dissolution was manifested as an increase in the flow cytometric parameters 90 degree scatter and fluorescein isothiocyanate fluorescence and was confirmed by light microscopy. (Copyright (c) Elsevier Scientific Publishers Ireland, Ltd.) |
| Supplementary Notes |
Pub. in Toxicology, v53 p69-78 Jul 88. Prepared in cooperation with NSI Technology Services Corp., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Metal-Induced Alteration of the Cell Membrane/Cytoplasm Complex Studied by Flow Cytometry and Detergent Lysis. |
| Category Codes |
57Y; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
013024586 |
| Origin |
NTIS |
| Type |
CAT |
