| Main Title |
Flow Cytometric Analysis of the Cellular Toxicity Tributyltin. |
| Author |
Zucker, R. M. ;
Elstein, K. H. ;
Easterling, R. E. ;
Massaro., E. J. ;
|
| CORP Author |
Harry G. Armstrong Aerospace Medical Research Lab., Wright-Patterson AFB, OH. ;NSI Technology Services Corp., Dayton, OH. ;Naval Medical Research Inst., Bethesda, MD. ;Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
| Publisher |
Nov 87 |
| Year Published |
1987 |
| Report Number |
EPA/600/D-90/027; |
| Stock Number |
PB90-246117 |
| Additional Subjects |
Proceedings ;
Toxicology ;
Fluorescence ;
Deoxyribonucleic acids ;
Tributyltin ;
Cell survival ;
Flow cytometry ;
Cell membrane ;
Cultured cells ;
Membrane potential ;
Cell differentiation ;
Dose-response relationships
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-246117 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
24p |
| Abstract |
Flow cytometric and light/fluorescence microscopic analyses indicate that tributyltin (TBT) alters the plasma membrane/cytoplasm complex of the murine erythroleukemic cell (MELC) in a dose dependent and time-dependent manner. The flow cytometric parameter axial light loss, a measure of cell volume, decreases in cells exposed to 5 micro M TBT relative to control cells or cells exposed to 50 micro M TBT. The flow cytometric parameter 90 deg light scatter, a function of refractive index and a measure of protein content, increases as a function of TBT concentration above 0.5 micro M, but less than 50 micro M DNA distribution across the cell cycle cannot be resolved adequately by flow cytometry. Relative to logarithmically growing cells, MELC in the stationary phase of the growth cycle and butyric acid-differentiated cells exhibit decreased plasma membrane permeability resulting in increased carboxyfluorescein (CF) retention derived from the intracellular hydrolysis of carboxyfluorescein diacetate (CFDA). Similarly, cells exposed to TBT concentrations below 50 micro M exhibit increased cellular CF retention. Viability in terms of CFDA hydrolysis/CF retention and propidium iodide (PI) exclusion is not decreased by exposure to TBT concentrations below 1 micro M. At doses between 5 and 50 micro M, however, cells exhibit both CF and PI fluorescence simultaneously and are programmed for death. |
| Supplementary Notes |
See also PB90-186263. Proceedings of the Conference on Toxicology (17th) held on November 3-5, 1987. Prepared in cooperation with NSI Technology Services Corp., Dayton, OH., Naval Medical Research Inst., Bethesda, MD., and Northrop Services, Inc., Research Triangle Park, NC. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| Category Codes |
57Y |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
024228658 |
| Origin |
NTIS |
| Type |
CAT |
