| Main Title |
Development of a carcinogen assay system utilizing estuarine fishes |
| Author |
Martin, B. J.
|
| CORP Author |
University of Southern Mississippi, Hattiesburg. Dept. of Biology.;Environmental Research Lab., Gulf Breeze, FL. |
| Publisher |
U.S. Environmental Protection Agency, Environmental Research Laboratory ; |
| Year Published |
1983 |
| Report Number |
EPA-600/3-82-091. |
| Stock Number |
PB83-136333 |
| OCLC Number |
10236602 |
| Subjects |
Carcinogenicity testing ;
Fishes--Effect of water pollution on ;
Biological assay
|
| Additional Subjects |
Bioassay ;
Carcinogens ;
Toxicology ;
Development ;
Lethal dosage ;
Fishes ;
Estuaries ;
Contaminants ;
Liver ;
Exposure ;
Concentration(Composition) ;
Anomalies ;
Cyprinodon variegatus ;
Benzidines ;
Cell lines ;
Archosargus probatocephalus ;
Telecosts ;
Toxic substances ;
Benzopyrenes
|
| Internet Access |
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| EKCD |
EPA 600-3-82-091 |
|
CEMM/GEMMD Library/Gulf Breeze,FL |
02/08/2008 |
| NTIS |
PB83-136333 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
x, 50 p. : ill. ; 28 cm. |
| Abstract |
The objective of this project was the development of systems to assay the effects of chemical carcinogens on marine teleosts. It was determined that the LC-50 for benzidine with respect to Cyprinodon variegatus was ca. 64 ppm. Weekly contaminations of 1 ppm benzidine caused some individuals to develop proliferative liver lesions. Exposure of C. variegatus early embroyos produced the following anomalies at concentrations of 50 ppm and above: tubed heart syndrome with distended pericardia, poor circulation, sparse distribution of melanophores, inability to hatch, abnormal head morphology, scoliosis, and faint RBC pigmentation. Chronic exposure of a cell line from Archosargus probatocephalus to benzidine and benzo(a)pyrene produced mutagenic effects. Two novel techniques were developed to study the effects of carcinogens on C. variegatus at the cellular level -- an aseptic embryo technique and an embryo-primary cell culture technique. Standard immunological techniques were miniaturized to study the immune system of C. variegatus. Serum electrophoresis disclosed that the serum proteins of benzidine-exposed fish differed from unexposed controls. |
| Notes |
"November 1982." "EPA-600/3-82-091." Includes bibliographical references. |
| Place Published |
Gulf Breeze, FL : |
| Corporate Au Added Ent |
Environmental Research Laboratory (Gulf Breeze, Fla.) |
| PUB Date Free Form |
1982. |
| BIB Level |
m |
| Cataloging Source |
OCLC/T |
| OCLC Time Stamp |
20080204134041 |
| Language |
eng |
| Origin |
OCLC |
| Type |
MERGE |
| OCLC Rec Leader |
01022cam 2200277Ka 45020 |
