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OLS Field Name OLS Field Data
Main Title Benzoate-Dependent Induction from the OP2 Operator-Promoter Region of the TOL pWWO in the Absence of Known Plasmid Regulatory Genes (Journal Version).
Author Cuskey, S. M. ; Sprenkle, A. B. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Technical Resources, Inc., Gulf Breeze, FL.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/170;
Stock Number PB89-119432
Additional Subjects Enzyme induction ; Oxygenases ; Operon ; Microbial genetics ; Gene expression regulation ; Molecular cloning ; Genetic transformation ; Benzoates ; Plasmids ; Reprints ; Pseudomonas putida
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NTIS  PB89-119432 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/14/1989
Collation 7p
Abstract The OP2 (Pm) operator-promoter region of the TOL plasmid, pWWO, is located immediately upstream from the 'lower' pathway operon responsible for the catabolism of benzoate and related aromatic acids. Induction of the operon in the presence of aromatic acid inducers, requires the xylS gene product. The authors cloned a 401 base pair region containing the OP2 region upstream from the promoter less xylE (catechol 2,3-dioxygenase) gene and measured induction in the presence of a variety of aromatic inducers in a xylS free background. Approximately 30-fold induction was seen when transformed cells of Pseudomonas putida strain PRS2000 were grown on lactate in the presence of benzoate. Slight (4-fold) induction occurred when m-toluic acid was included in the growth medium.
Supplementary Notes Prepared in cooperation with Technical Resources, Inc., Gulf Breeze, FL.
Availability Notes Pub. in Jnl. of Bacteriology, v170 n8 p3742-3746 1988.
NTIS Title Notes Journal article.
Title Annotations Reprint: Benzoate-Dependent Induction from the OP2 Operator-Promoter Region of the TOL pWWO in the Absence of Known Plasmid Regulatory Genes (Journal Version).
Category Codes 57F; 57K; 57B
NTIS Prices Not available NTIS
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 901217142
Origin NTIS
Type CAT