||Sequence Similarities in the Genes Encoding Polychlorinated Biphenyl Degradation by 'Pseudomonas' Strain LB400 and 'Alcaligenes eutrophus' H850.
Yates, J. R. ;
Mondello, F. J. ;
||General Electric Co., Schenectady, NY. Research and Development Center.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Polychlorinated biphenyls ;
Bacterial genes ;
DNA hybridization ;
Cell constitutents ;
Genetic engineering ;
Pseudomonas strain LB400 ;
Alcaligenes eutrophus H850 ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||DNA-DNA hybridization was used to compare the Pseudomonas strain LB400 genes for polychlorinated biphenyl (PCB) degradation with those from seven other PCB-degrading strains. Significant hybridization was detected to the genome of Alcaligenes eutrophus H850, a strain similar to LB400 in PCB-degrading capability. These two organisms showed a strong conservation of restriction sites in the region of DNA encoding PCB metabolism. No other sequence similarities were detected in the two genomes. DNA from the other PCB-degrading strains showed no hybridization to the probe, which demonstrated the existence of at least two distinct classes of genes encoding PCB degradation. (Copyright (c) 1989, American Society for Microbiology.)
||Pub. in Jnl. of Bacteriology, v171 n3 p1733-1735 Mar 89. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
|NTIS Title Notes
||Reprint: Sequence Similarities in the Genes Encoding Polychlorinated Biphenyl Degradation by 'Pseudomonas' Strain LB400 and 'Alcaligenes eutrophus' H850.
||57K; 57F; 99F; 68
||PC A01/MF A01