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RECORD NUMBER: 369 OF 2019

Main Title Constitutive Degradation of Trichloroethylene by an Altered Bacterium in a Gas-Phase Bioreactor.
Author Shields, M. S. ; Reagin, M. J. ; Gerger, R. R. ; Somerville, C. ; Schaubhut, R. ;
CORP Author University of West Florida, Pensacola. ;Technical Resources, Inc., Gulf Breeze, FL.;Environmental Research Lab., Gulf Breeze, FL. Office of Research and Development.;SBP Technologies, Inc., Gulf Breeze, FL.;Department of the Air Force, Washington, DC.
Publisher 1994
Year Published 1994
Report Number EPA-68-03-3479 ;USAF-F-08635-92-C-0103; EPA/600/A-94/204;
Stock Number PB95-122941
Additional Subjects Water pollution control ; Bioreactors ; Bacteria ; Waste water treatment ; E coli ; Pseudomonas ; Biodegradation ; Toluene ; Chemical analysis ; Biotechnology ; Ground water ; Aerobic conditions ; Chlorinated aliphatic hydrocarbons ; Reprints ; Ethylene/trichloro ; TOM(Toluene orth-monooxygenase) ; TCE(Trichloroethylene)
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NTIS  PB95-122941 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 20p
Abstract Pseudomonas cepacia G4 expresses a unique toluene orth-monooxygenase (Tom) that enables it to degrade toluene and trichloroethylene (TCE). Transposon mutants of G4 have been isolated that constitutively express Tom. Two fixed-film bioreactor designs were investigated for the exploitation of one such constitutive strain (G4 PR1) in the degradation of vapor-phase TCE. DNA probe analysis indicates that the genes responsible for the toluene catabolic pathway are located on a large plasmid of G4 (> or = 150 kb) termed pG4L. The authors propose that pG4L will serve as a archetype for a new class of catabolic plasmid known as Tom, which encodes an ortho-hydroxylation pathway for the degradation of benzene, toluene, o-xylene, cresols, and phenol.
Supplementary Notes Pub. in Bioremediation of Chlorinated and Polycyclic Aromatic Hydrocarbon Compounds, p50-65 1994. Prepared in cooperation with Technical Resources, Inc., Gulf Breeze, FL. Sponsored by Environmental Research Lab., Gulf Breeze, FL. Office of Research and Development., SBP Technologies, Inc., Gulf Breeze, FL., and Department of the Air Force, Washington, DC.
Highlights Notes %AUT:R. /Campbell ;J. /Hu-Primmer
NTIS Title Notes Book chapter.
Title Annotations Reprint: Constitutive Degradation of Trichloroethylene by an Altered Bacterium in a Gas-Phase Bioreactor.
Category Codes 68D; 57K
NTIS Prices PC A03/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 433518507
Origin NTIS
Type CAT