| Main Title |
Biotransformation of Benzothiophene by Isopropylbenzene-Degrading Bacteria. |
| Author |
Eaton, R. W. ;
Nitterauer, J. D. ;
|
| CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Technical Resources, Inc., Gulf Breeze, FL. ;Arkansas Univ. for Medical Sciences, Little Rock. |
| Publisher |
c1994 |
| Year Published |
1994 |
| Report Number |
EPA/600/J-94/440; |
| Stock Number |
PB95-112199 |
| Additional Subjects |
Bacteria ;
Biodeterioration ;
Thiophene ;
Metabolites ;
Metabolism ;
Oxidation ;
Enzymes ;
Catalysis ;
Reaction kinetics ;
Petroleum industry ;
Organic sulfur compounds ;
Reprints ;
Thiophene/benzo
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB95-112199 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
13p |
| Abstract |
Isopryopylbenzene-degrading bacteria, including Pseudomonas putida RE204, transform benzothiophene to a mixture of compounds. Induced strain RE204 and a number of its Tn5 mutant derivatives were used to accumulate these compounds and their precursors from benzothiophene. These metabolites were subsequently identified by (1)H and (13)H nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. When strain RE204 was incubated with benzothiophene, it produced a bright yellow compound, identified as trans-4-(3-hydroxy-2-thienyl)-2-oxobut-3-enoate, formed by the rearrangement of cis-4-(3-deto-2,3-dihydrothienyl)-2-hydroxybuta-2,4-dienoate, the product of 3-isopropylcatechol-2,3-dioxygenase-catalyzed ring cleavage of 4,5-dihydroxybenzothiophene, as well as 2-mercaptophenylglyoxalate and 2'-mercaptomandelaldehyde. These data indicate that oxidation of benzothiophene by strain RE204 is initiated at either ring. |
| Supplementary Notes |
Pub. in Jnl. of Bacteriology, v176 n13 p3992-4002 Jul 94. Prepared in cooperation with Technical Resources, Inc., Gulf Breeze, FL. and Arkansas Univ. for Medical Sciences, Little Rock. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Biotransformation of Benzothiophene by Isopropylbenzene-Degrading Bacteria. |
| Category Codes |
97K; 97R; 57K; 68A |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
433518115 |
| Origin |
NTIS |
| Type |
CAT |
