| Main Title |
Assessing the Effect of Colony Counting Methods and Genetic Drift on Ames Bioassay Results. |
| Author |
Claxton, L. D. ;
Toney, S. ;
Perry, E. ;
King, L. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. |
| Year Published |
1984 |
| Report Number |
EPA/600/J-84/083; |
| Stock Number |
PB84-244151 |
| Additional Subjects |
Counting ;
Bacteria ;
Salmonella ;
Bioassay ;
Assessments ;
Reprints ;
Ames test ;
Colony counting
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB84-244151 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
15p |
| Abstract |
Accuracy of automatic colony counters and variation among subcultures of the Salmonella tester strains are two factors that cause variation within the Ames bioassay. This paper examines these potential sources of variation. In contrast to results seen by other researchers, this genetic drift study demonstrated that except for TA100 the subcultures of tester strains from four research laboratories are very stable in biochemical characteristics measured using Analytical Profile Index (API) strips. This study also documented the levels of accuracy for hand- and automatic-counting techniques, and provides a simple method for generating acetate calibration transparencies for use with most colony counters. |
| Supplementary Notes |
Prepared in cooperation with Northrop Services, Inc., Research Triangle Park, NC. |
| Availability Notes |
Pub. in Environmental Mutagenesis 6, p331-342 1984. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Assessing the Effect of Colony Counting Methods and Genetic Drift on Ames Bioassay Results. |
| PUB Date Free Form |
c1984 |
| Category Codes |
6M; 57K |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
435625655 |
| Origin |
NTIS |
| Type |
CAT |
