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Main Title Nutritional Requirements of the Submerged Angio-Sperm 'Ruppia maritima' in Algae-Free Culture.
Author Thursby, G. B. ;
CORP Author Environmental Research Lab., Narragansett, RI. ;Rhode Island Univ., Kingston. Dept. of Botany.
Year Published 1984
Report Number EPA-600/J-84-047 ;CONTRIB-580;
Stock Number PB84-222660
Additional Subjects Aquatic plants ; Plant nutrition ; Growth ; Brackish water ; Vitamins ; Metals ; Iron ; Inorganic phosphates ; Concentration(Composition) ; Inorganic nitrates ; Tissues(Biology) ; Requirements ; Reprints ; Ruppia maritima ; Vascular plants
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NTIS  PB84-222660 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Abstract Ruppia maritima has the potential to become a model laboratory organism for studies with submerged aquatic vascular plants. The present study demonstrated that algae-free R. maritima grew well in a defined medium without sediment. Growth was a linear response to photon flux density over the range of light tested. Vitamins may be a necessary addition in artificial seawater. Trace metals caused little or no increase in growth during short-term (3 wk) growth studies, but appear to be required for long-term cultivation. Iron also caused no increase in growth, at the concentrations tested, but plants were greener in 1.46 micrometers M iron. A nitrate concentration of 110 micrometers M and a phosphate concentration of 2.3 micrometers M were sufficient for maximum growth. However, 4.5 micrometers M phosphate eliminated occasional CaCO3 precipitation in stock cultures. Critical tissue nitrogen content was between 2.5 and 3.0%, and critical phosphorus content between 0.25 and 0.35%. A comparison with field data suggests that R. maritima was deficient in both nitrogen and phosphorus during much of the summer.
Supplementary Notes Prepared in cooperation with Rhode Island Univ., Kingston. Dept. of Botany.
Availability Notes Pub. in Marine Ecology Progress Series, v16 p45-50 1984.
NTIS Title Notes Journal article.
Title Annotations Reprint: Nutritional Requirements of the Submerged Angio-Sperm 'Ruppia maritima' in Algae-Free Culture.
PUB Date Free Form c1984
Category Codes 8A; 47D
NTIS Prices Not available NTIS
Primary Description 600/05
Document Type NT
Cataloging Source NTIS/MT
Control Number 428625133
Origin NTIS
Type CAT