Main Title |
Use of Amphotericin B for Differentiating Eukaryotic from Prokaryotic Activity in Mixed Microbial Samples. |
Author |
Lewis, D. L. ;
Hamala, J. A. ;
|
CORP Author |
Environmental Research Lab., Athens, GA. ;Technology Applications, Inc., Athens, GA. |
Year Published |
1987 |
Report Number |
EPA/600/J-87/205; |
Stock Number |
PB88-170535 |
Additional Subjects |
Antibiotics ;
Bacteria ;
Algae ;
Fungi ;
Reprints ;
Amphotericin B ;
Prokaryotes ;
Eukaryotes
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB88-170535 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
In axenic and mixed cultures, amphotericin B (25-100 mg/L) was tested for inhibition of selected metabolic activities of algae, fungi, bacteria and zooplankton. Effects of the antibiotic were determined based on inhibition of 14-C-labeled bicarbonate uptake, hydrolysis of the methyl ester of 2,4-dichlorophenoxyacetic acid, growth and acid production. Amphotericin B was dissolved in water by either hydrolyzing the antibiotic or mixing it with sodium deoxycholate. Treatments of mixed suspended microbial communities with 200 mg/L of amphotericin B for at least 15 min was effective for differentiating prokaryotic (bacterial) from eukaryotic (algae and fungi, but not zooplankton) activities. |
Supplementary Notes |
Pub. in Jnl. of Microbiological Methods 6, p257-264 1987. Prepared in cooperation with Technology Applications, Inc., Athens, GA. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Use of Amphotericin B for Differentiating Eukaryotic from Prokaryotic Activity in Mixed Microbial Samples. |
PUB Date Free Form |
c1987 |
Category Codes |
57K |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/01 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
812722425 |
Origin |
NTIS |
Type |
CAT |