||Mutagenicity Evaluation of Chlorendic Anhydride in the Unscheduled DNA Synthesis in Human WI-38 Cells Assay (Final Report) with Attachments and Cover Letter Dated 06/15/78.
||Litton Bionetics, Inc., Kensington, MD. ;Velsicol Chemical Corp., Chicago, IL.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
||28 Jun 1978
Deoxyribonucleic acid ;
Cell cultures ;
Molecular biology ;
Chlorendic anhydride ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||The test material was evaluated for its ability to induce unscheduled DNA synthesis in human WI-38 cells blocked in G1 phase. The results area presented in Tables 1 and 2. The test substance was prepared as a stock solution in DMSO. The stock solution was diluted in culture medium to derive the final test concentrations. The range of concentrations employed in this assay were from 0.01 mg/ml to 0.5 mg/ml. The only evidence of toxicity was observed at the highest dose level of 0.5 mg/ml. The evidence of toxicity was derived from the reduction in counts (CPM) below the control. The results of the test are shown in Tables 1 and 2. They are presented in tabular and graphic forms. The data from this assay showed significant increases in UDS both with and without a microsome S9 preparation. The activation test conditions provided the greatest increases over control.
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||PC A05/MF A01