||Determination of Mineral Fiber Concentrations in Fish Tissues.
Batterman, Allan R. ;
Cook, Philip M. ;
||Environmental Research Lab.-Duluth, MN.
Mineral fibers ;
Electron microscopy ;
Lake trout ;
Water pollution effects(Animals)
||Some EPA libraries have a fiche copy filed under the call number shown.
||Submicroscopic inorganic particle concentrations in tissue have not been quantitatively determined in the past because of a lack of sample preparation techniques capable of achieving the sensitivity required. The determination of whether mineral fibres in water are accumulated in aquatic organisms requires transmission electron microscope examination of bulk tissue residues rather than thin sections. The sample preparation method used for this investigation involved removal of water and organic matter by freeze-drying and low temperature ashing. Lake trout with a lifetime exposure to Lake Superior water containing amphibole fibers contained similar amphibole fibers particularly in the kidney and with low concentrations in muscle tissue. Lake trout from two locations with widely different water fiber concentrations had corresponding differences in tissue fiber concentrations. Analysis of other fish raised under laboratory conditions suggests that ingestion is the primary route for fiber accumulation.
||Pub. in Canadian Jnl. of Fisheries and Aquatic Science, v38 p952-959 1981. Summary in English and French.
|NTIS Title Notes
||Reprint: Determination of Mineral Fiber Concentrations in Fish Tissues.
|PUB Date Free Form
||24 Apr 81
||6T; 6F; 57Y; 68D
||PC A02/MF A01