Main Title |
Structure and Expression of the Genes, mcrBDCGA, which Encode the Subunits of Component C of Methyl Coenzyme M Reductase in 'Methanococcus vannielii'. |
Author |
Reeve, J. N. ;
Cram, D. S. ;
Sherf, B. A. ;
Libby, R. T. ;
Mattaliano, J. ;
|
CORP Author |
Ohio State Univ., Columbus. Dept. of Microbiology. ;Biogen Research Corp., Cambridge, MA.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
Publisher |
c1987 |
Year Published |
1987 |
Report Number |
EPA-R-812774; EPA/600/J-87/494; |
Stock Number |
PB90-106287 |
Additional Subjects |
Deoxyribonucleic acids ;
Peptides ;
Reprints ;
Methanococcus vannielii ;
Archaebacteria ;
Bacterial genes ;
Methyl coenzyme M reductase ;
Gene expression regulation ;
Codon ;
Genetic translation ;
Base sequence ;
Amino acid sequence ;
Molecular cloning
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Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-106287 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
8p |
Abstract |
The genes that encode the alpha, beta, and gamma subunits of component C of methyl coenzyme M reductase (mcrA, mcrB, and mcrG) in Methanococcus vannielii have been cloned and sequenced, and their expression in Escherichia coli has been demonstrated. These genes are organized into a five-gene cluster, mcrBDCGA, which contains two genes, designated mcrC and mcrD, with unknown functions. The mcr genes are separated by very short intergenic regions that contain multiple translation stop codons and strong ribosome binding sequences. Although the genome of M. vannielii is 69 mol % A+T, there is a very strong preference in the mcrA, mcrB, and mcrG genes for the codon with a C in the wobble position in the codon pairs AA(C/U) UU(C/U) (phenylalanine), and UA(C/U) (tyrosine). The mcrC and mcrD genes do not show this codon preference and frequently have U or A in the wobble position. As the codon pairs listed above are likely to be translated by the same tRNA with a G in the first anticodon position, the presence of C in the wobble position might ensure maximum efficiency of translation of transcripts of these very highly expressed genes. |
Supplementary Notes |
Pub. in Proceedings of National Academy of Science USA, v84 p3992-3996 1987. Prepared in cooperation with Biogen Research Corp., Cambridge, MA. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Structure and Expression of the Genes, mcrBDCGA, which Encode the Subunits of Component C of Methyl Coenzyme M Reductase in 'Methanococcus vannielii'. |
Category Codes |
57F; 57K; 68G |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/14 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
932619230 |
Origin |
NTIS |
Type |
CAT |