| Main Title |
Application of the Micro-Forward Mutation Assay to Assess Mutagenicity of Airborne Particulates in Indoor. |
| Author |
Takagi, Y. ;
Goto, S. ;
Murata, M. ;
Matsushita, H. ;
Lewtas., J. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
| Publisher |
c1988 |
| Year Published |
1988 |
| Report Number |
EPA/600/J-88/542; |
| Stock Number |
PB91-109231 |
| Additional Subjects |
Air pollution ;
Toxicity ;
Mutations ;
Particle size ;
Salmonella typhimurium ;
Benzene ;
Ethyl alcohol ;
Foreign technology ;
Mutagenicity test ;
Indoor air pollution ;
Metabolic activation
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-109231 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
13p |
| Abstract |
A validity test of the micro-forward mutation assay using Salmonella typhimurium strain TM677 was carried out using benzene-ethanol extracts from airborne particulates as test materials. Sensitivity of this assay in the presence and absence of S-9 mix was five to ten times higher than the pre-incubation method, a modified method of Ames's original method, using Salmonella typhimurium strains TA98 and TA100. This means that the virtual sensitivity of the micro-forward mutation assay is greater by ten times or more than the pre-incubation method, because two or more strains are necessary in the latter method. Repeatability of the micro-forward mutation assay was nearly same with that of the pre-incubation method. That is, the coefficient of variation of mutagenic frequency of the particulate extracts was about 10%. This forward mutation assay was applied to airborne particulates indoors, and proved to be able to measure easily the mutagenic activity of the extracts from particulates collected by 30 cu m-air sampling. Some indoor pollutants, especially ones collected in a room in which cigarette smoking was done, showed higher mutagenic activity than that of outdoor sample. |
| Supplementary Notes |
Text in Japanese and English. Pub. in Jnl. Japan Soc. Air Pollut., v23 p24-31 1988. Portions of this document are not fully legible. |
| NTIS Title Notes |
Journal article. |
| Category Codes |
57Y; 57F; 68A |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
101122697 |
| Origin |
NTIS |
| Type |
CAT |
