| Main Title |
Reactivation and Aging of Phosphorylated Brain Acetylcholinesterase from Fish and Rodents. |
| Author |
Wallace, K. B. ;
Herzberg, U. ;
|
| CORP Author |
Minnesota Univ.-Duluth. Dept. of Pharmacology.;Environmental Research Lab.-Duluth, MN. |
| Publisher |
c1988 |
| Year Published |
1988 |
| Report Number |
EPA-R-810963; EPA/600/J-88/453; |
| Stock Number |
PB90-147364 |
| Additional Subjects |
Brain ;
Acetylcholinesterase ;
Phosphorylation ;
Aging(Biology) ;
Toxicity ;
Cholinesterase inhibitors ;
Exposure ;
In vitro analysis ;
Rodents ;
Aquatic animals ;
Reprints ;
Pharmacokinetics ;
Dose-response relationships
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-147364 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Species-related differences in sensitivity to acute intoxication by anticholinesterase compounds have been attributed, in large part, to differences in the kinetics of inhibition of acetylcholinesterase (AChE) in vitro. Since inhibition of AChE is also influenced by the stability of the phosphorylated enzyme complex, it was of interest to compare the rates at which the inhibited enzyme from different species subsequently either reactivates or ages. Brain AChE from rats, mice, fathead minnows, or rainbow trout was preincubated with an IC90 concentration of either paraoxon or malaoxon. The first-order rate constants for both the reactivation and aging of paraoxon-inhibited AChE from rats and mice were significantly greater than those observed for either species of fish. Following malaoxon inhibition, however, rodent AChE reactivated more rapidly but aged more slowly than did the enzyme from minnows. Therefore, the data suggest that compared to rodents, intermittent or continuous exposure of fish to sublethal concentrations of anticholinesterase compounds is more likely to result in a cumulative toxicity owing to the relative irreversibility of AChE inhibition. (Copyright (c) 1988 Academic Press, Inc.) |
| Supplementary Notes |
Pub. in Toxicology and Applied Pharmacology, v92 p307-314 1988. Presented at the Annual Meeting of the Society of Toxicology, Washington, DC., 1987. Sponsored by Environmental Research Lab.-Duluth, MN. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Reactivation and Aging of Phosphorylated Brain Acetylcholinesterase from Fish and Rodents. |
| Category Codes |
57Y; 57S |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/03 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
007531085 |
| Origin |
NTIS |
| Type |
CAT |
