| Main Title |
Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Membrane-Thermotolerant Escherichia coli Agar (mTEC), March 2010. |
| CORP Author |
Environmental Protection Agency, Washington, DC. Office of Water. |
| Year Published |
2010 |
| Report Number |
EPA/821/R-10/002 |
| Stock Number |
PB2010-114246 |
| Additional Subjects |
Escherichia coli ;
Membrane filtration ;
Water sampling ;
Bacteria ;
Agar ;
Enumeration ;
Epidemiology ;
Microorganisms ;
Criteria ;
Water quality ;
Marine environment ;
Health effects ;
Gastroenteristis ;
Feces ;
Water pollution effects ;
Surface waters ;
Counting methods ;
Calibration ;
Growth(Biology) ;
Contamination ;
Quality control ;
Pollution prevention ;
Laboratories ;
Test methods
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB2010-114246 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
46p |
| Abstract |
The MF method provides a direct count of bacteria in water based on the development of colonies on the surface of the membrane filter (Reference 18.4). A water sample is filtered through the membrane which retains the bacteria. After filtration, the membrane is placed on a selective and differential medium, mTEC, incubated at 35 degrees C + or = 0.5 degrees C for 2 + or = 0.5 hours to resuscitate injured or stressed bacteria, and then incubated at 44.5 degrees C + or = 0.2 degrees C for 22 + or = 2 hours. Following incubation, the filter is transferred to a filter pad saturated with urea substrate. After 15 minutes, yellow, yellow-green, or yellow-brown colonies are counted with the aid of a fluorescent lamp and a magnifying lens. |
| Supplementary Notes |
See also PB2007-101966, July 2006. |
| Availability Notes |
Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5301 Shawnee Road, Alexandria, VA, 22312, USA. |
| PUB Date Free Form |
Mar 2010 |
| Category Codes |
57K; 57U; 68D |
| NTIS Prices |
PC A04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
128601780 |
| Origin |
NTIS |
| Type |
CAT |
