| Main Title |
Reversibility of Tributyltin-Induced Toxicity In vitro as a Function of Concentration and Duration of Exposure (C X T). |
| Author |
Zucker, R. W. ;
Massaro, E. J. ;
Elstein, K. H. ;
|
| CORP Author |
NSI Technology Services Corp., Research Triangle Park, NC. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA-68-02-4450; EPA/600/J-92/115; |
| Stock Number |
PB92-164854 |
| Additional Subjects |
Toxicity ;
Dose-response relationships ;
Pesticides ;
Cell membrane permeability ;
Exposure ;
Cytoplasm ;
Fluorescence ;
S phase ;
Cell cycle ;
Acute erythroblastic leukemia ;
Deoxyribonucleic acids ;
Reprints ;
Tributyltin
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-164854 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
The toxicity exhibited by murine erythroleukemic cells (MELC) exposed to tributyltin (TBT) is a function of both concentration (C) and duration of exposure (T). At or above a critical C x T product value (CPV), exposed MELC exhibit severe, irreversible toxicity: decreased membrane integrity (viability, measured by propidium iodide (PI) exclusion), grossly perturbed cell cycle distributions, and fixation of the plasma membrane/cytoplasm complex. Below the CPV, exposed cells exhibit retention of carboxyfluorescein (CF) fluorescence (indicative of decreased plasma membrane permeability) and decreased cell proliferation, a result of retardation of progression into, through, and out of the S (DNA synthetic) phase of the cell cycle. However, following washout and recovery, mean CF fluorescence, cell proliferative capacity, and cell-cycle kinetics return to control levels. These results suggest that the toxic changes induced by TBT exposure may be reversible if exposure conditions do not exceed the CPV. |
| Supplementary Notes |
Pub. in Environmental Research, v57 n1 p107-116 Feb 92. See also PB89-218192. Prepared in cooperation with ManTech Environmental Technology, Inc., Research Triangle Park, NC. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Reversibility of Tributyltin-Induced Toxicity In vitro as a Function of Concentration and Duration of Exposure (C X T). |
| Category Codes |
57Y; 57P; 68G |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
214321474 |
| Origin |
NTIS |
| Type |
CAT |
