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RECORD NUMBER: 210 OF 956

Main Title Development of Immobilized Enzyme Systems for Enhancement of Biological Waste Treatment Processes.
Author Slot, Lawrence ;
CORP Author Grumman Aerospace Corp., Bethpage, N.Y.
Year Published 1970
Report Number DI-14-12-562; EPA-WQO-16050-DXN; 00002,; 16050-DXN-07/70
Stock Number PB-203 598
Additional Subjects ( Sewage treatment ; Activated sludge process) ; ( Enzymes ; Activated sludge process) ; Separation ; Lagoons(Ponds) ; Colloids ; Gels ; Bacteria ; Aeration ; Ribonucleic acids ; Amylase ; Lipase ; Polyacrylamides ;
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB-203 598 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 67p
Abstract Degradation and utilization of waste water nutrients by microbial action is controlled by a complex enzyme system. In attempts to produce a faster-acting system, a method was developed to biochemically fractionate the microbial enzymes from activated sludge, to concentrate and characterize their activity, and to immobilize this activity by entrapment in a polyacrylamide gel. The enzyme gel preparation was tested for its effect on the biological degradation of a bench-scale activated sludge process. Soluble enzymatic components were readily separated from particulate cell components, and the remaining soluble system was fractioned such that catabolic activity in the enzyme systems of interest was maintained while non-essential components were removed. Immobilization within the gel allowed activity to be maintained during repeated washings, exposure to substrate, and storage. Limited bench-scale tests produced no meaningful results possibly on account of either incomplete polymerization of the gel, an improper activated sludge culture, or both.
Supplementary Notes Paper copy available from GPO $0.70 as SN-5501-0113.
PUB Date Free Form Jul 70,
Category Codes 13B; 68D
NTIS Prices PC-GPO/MF A01-NTIS
Document Type NT
Cataloging Source NTIS/MT
Control Number 326522197
Origin NTIS
Type CAT