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Main Title Fertilization by Sperm Microinjection and Zona Drilling: Applications in the Basic and Clinical Sciences.
Author Perreault, S. D. ; Naish, S. J. ; Zirkin, B. R. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;California Univ., Santa Barbara. Dept. of Biological Sciences. ;Johns Hopkins Univ., Baltimore, MD. Dept. of Population Dynamics.
Year Published 1988
Report Number EPA/600/D-88/096;
Stock Number PB88-211784
Additional Subjects Reproduction(Biology) ; Fertility ; Sperm microinjection ; Zona drilling method ; Sperm ; Eggs
Library Call Number Additional Info Location Last
NTIS  PB88-211784 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 25p
Abstract Experimental manipulation of fertilization provides an exciting research approach for studying mechanisms involved in sperm/egg interaction and holds great promise as a means for overcoming some forms of human infertility. The report will focus on three methods for assisted fertilization: (1) micro-injection of a spermatozoon (or sperm nucleus) directly into the ooplasm; (2) injection of motile sperm through the zona pellucida and into the perivitelline space (PVS); and (3) zona-drilling or the creation of a channel in the zona pellucida through which sperm may contact the oocyte membrane directly. The use of these methods to address a variety of questions in gamete and developmental biology, as well as to initiate embryonic development in animals and humans, will be reviewed.
Supplementary Notes See also PB88-211776. Prepared in cooperation with California Univ., Santa Barbara. Dept. of Biological Sciences, and Johns Hopkins Univ., Baltimore, MD. Dept. of Population Dynamics.
PUB Date Free Form May 88
Category Codes 57E#
NTIS Prices PC A03/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 821127506
Origin NTIS
Type CAT