| Main Title |
Maintenance and Stability of Introduced Genotypes in Groundwater Aquifer Material. |
| Author |
Jain, R. K. ;
Sayler, G. S. ;
Wilson, J. T. ;
Houston, L. ;
Pacia, D. ;
|
| CORP Author |
Tennessee Univ., Knoxville.;Robert S. Kerr Environmental Research Lab., Ada, OK. |
| Year Published |
1987 |
| Report Number |
EPA-R-810485; EPA/600/J-87/136; |
| Stock Number |
PB88-148192 |
| Additional Subjects |
Groundwater ;
Biodeterioration ;
Aquifers ;
Microbiology ;
Pseudomonas ;
Contaminants ;
Maintenance ;
Stability ;
Organic wastes ;
Reprints ;
Genotype ;
Plasmids
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB88-148192 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Three indigenous groundwater bacterial strains and Pseudomonas putida harboring plasmids TOL (pWWO) and RK2 were introduced into experimentally contaminated groundwater aquifer microcosms. Maintenance of the introduced genotypes was measured over time by colony hybridization with gene probes of various specificity. On the basis of the results of colony hybridization quantitation of the introduced organisms and genes, all introduced genotypes were stably maintained at approximately 100,000 positive hybrid colonies g-1 of aquifer microcosm material throughout an 8-week incubation period. Concomitant removal of the environmental contaminants, viz., toluene, chlorobenzene, and styrene, in both natural (uninoculated) and inoculated aquifer microcosms was also demonstrated. The results indicate that introduced catabolic plasmids, as well as indigenous organisms, can be stably maintained in groundwater aquifer material without specific selective pressure for the introduced genotypes. These results have positive implications for in situ treatment and biodegradation in contaminated aerobic groundwater aquifers. (Copyright (c) Applied and Environmental Microbiology, 1987.) |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v53 n5 p996-1002 May 87. Sponsored by Robert S. Kerr Environmental Research Lab., Ada, OK. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Maintenance and Stability of Introduced Genotypes in Groundwater Aquifer Material. |
| PUB Date Free Form |
c1987 |
| Category Codes |
57K; 57F; 48G; 68D |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/15 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
808420099 |
| Origin |
NTIS |
| Type |
CAT |
