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OLS Field Name OLS Field Data
Main Title A fluorescence immunoassay technique for detecting organic environimental contaminants /
Author Lukens, Herbert R. ; Williams, Colin B. ; Levison, Stuart A. ; Dandliker, Walter B. ; Muryama., Dennis
Other Authors
Author Title of a Work
Williams, Colin B.
Levison, Stuart A.
Dandliker, Walter B.
Muryama, Dennis.
CORP Author IRT Corp., San Diego, Calif.;National Environmental Research Center, Research Triangle Park, N.C.;Scripps Clinic and Research Foundation, La Jolla, Calif.
Publisher U.S. Environmental Protection Agency, Pesticides and Toxic Substances Effects Laboratory,
Year Published 1975
Report Number EPA/650-1-75-004; INTEL-RT-8098-004; EPA-68-02-1266
Stock Number PB-243 397
OCLC Number 43079317
Subjects Immunofluorescence. ; Immunoassay. ; Fluorescent antigen technique. ; Fluorescent antibody technique.
Additional Subjects Contaminants ; Benzimidazoles ; Air pollution ; Water pollution ; Environments ; Organic compounds ; Antigen antibody reactions ; Chemical compounds ; Assaying ; Immunology ; Identifying ; Pesticides ; Pollutant identification ; Pesticide residues ; Immunofluorescence
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 650-1-75-004 Headquarters Library/Washington,DC 09/09/2013
ELBD RPS EPA 650-1-75-004 repository copy AWBERC Library/Cincinnati,OH 08/31/2020
NTIS  PB-243 397 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 01/01/1988
Collation xii, 63 pages ; 28 cm.
Abstract The report describes the development and successful demonstration of an immunological assay for the detection of low molecular weight organic contaminants of environmental cocern. The specific technique described is a fluorescence polarization immunoassay, the theory of which is presented. The preparation of the two required reagents, namely a fluorescent conjugate of the contaminant of interest, together with an antibody to the contaminant, is described in detail. The specific contaminant chosen for this study was 2-aminobenzimidazole (MW = 133), a metabolite of certain fungicide agents used in agriculture. The particular fluorescent moiety chosen to form the conjugate with 2-aminobenzimidazole with fluorescein. A successful demonstration of the assay has been accomplished, and a detection sensitivity in the sub-nanogram/ml range obtained.
Notes "EPA/650-1-75-004." "May 1975." EPA contract no. 68-02-1266, ROAP No. 21AFM, program element no. 1E1078; EPA project officer: Dr. Ronald L. Baron. Includes bibliographical references (page 35).
Place Published Research Triangle Park, NC
Supplementary Notes Prepared in cooperation with Scripps Clinic and Research Foundation, La Jolla, Calif.
Corporate Au Added Ent Pesticides and Toxic Substances Effects Research Laboratory (Research Triangle Park, N.C.); United States. Environmental Protection Agency. Office of Research and Development.; IRT Corporation.; Scripps Clinic and Research Foundation.
Title Ser Add Ent Research reporting series. Environmental health effects research ; EPA 650/1-75/004.
PUB Date Free Form May 1975
Series Title Untraced Environmental health effects research series ; EPA 650/1-75/004
NTIS Prices PC A04/MF A01
BIB Level m
Medium unmediated
Content text
Carrier volume
Cataloging Source OCLC/T
OCLC Time Stamp 20200818081634
Language eng
Origin OCLC
OCLC Rec Leader 02404cam 2200553Ka 45010