| Main Title |
A fluorescence immunoassay technique for detecting organic environimental contaminants / |
| Author |
Lukens, Herbert R. ;
Williams, Colin B. ;
Levison, Stuart A. ;
Dandliker, Walter B. ;
Muryama., Dennis
|
| Other Authors |
|
| CORP Author |
IRT Corp., San Diego, Calif.;National Environmental Research Center, Research Triangle Park, N.C.;Scripps Clinic and Research Foundation, La Jolla, Calif. |
| Publisher |
U.S. Environmental Protection Agency, Pesticides and Toxic Substances Effects Laboratory, |
| Year Published |
1975 |
| Report Number |
EPA/650-1-75-004; INTEL-RT-8098-004; EPA-68-02-1266 |
| Stock Number |
PB-243 397 |
| OCLC Number |
43079317 |
| Subjects |
Immunofluorescence ;
Immunoassay ;
Fluorescent antigen technique ;
Fluorescent antibody technique
|
| Additional Subjects |
Contaminants ;
Benzimidazoles ;
Air pollution ;
Water pollution ;
Environments ;
Organic compounds ;
Antigen antibody reactions ;
Chemical compounds ;
Assaying ;
Immunology ;
Identifying ;
Pesticides ;
Pollutant identification ;
Pesticide residues ;
Immunofluorescence
|
| Internet Access |
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| ELBD ARCHIVE |
EPA 650-1-75-004 |
Received from HQ |
AWBERC Library/Cincinnati,OH |
10/04/2023 |
| ELBD RPS |
EPA 650-1-75-004 |
repository copy |
AWBERC Library/Cincinnati,OH |
08/31/2020 |
| NTIS |
PB-243 397 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
xii, 63 pages ; 28 cm. |
| Abstract |
The report describes the development and successful demonstration of an immunological assay for the detection of low molecular weight organic contaminants of environmental cocern. The specific technique described is a fluorescence polarization immunoassay, the theory of which is presented. The preparation of the two required reagents, namely a fluorescent conjugate of the contaminant of interest, together with an antibody to the contaminant, is described in detail. The specific contaminant chosen for this study was 2-aminobenzimidazole (MW = 133), a metabolite of certain fungicide agents used in agriculture. The particular fluorescent moiety chosen to form the conjugate with 2-aminobenzimidazole with fluorescein. A successful demonstration of the assay has been accomplished, and a detection sensitivity in the sub-nanogram/ml range obtained. |
| Notes |
"EPA/650-1-75-004." "May 1975." EPA contract no. 68-02-1266, ROAP No. 21AFM, program element no. 1E1078; EPA project officer: Dr. Ronald L. Baron. Includes bibliographical references (page 35). |
| Place Published |
Research Triangle Park, NC |
| Supplementary Notes |
Prepared in cooperation with Scripps Clinic and Research Foundation, La Jolla, Calif. |
| Corporate Au Added Ent |
Pesticides and Toxic Substances Effects Research Laboratory (Research Triangle Park, N.C.); United States. Environmental Protection Agency. Office of Research and Development.; IRT Corporation.; Scripps Clinic and Research Foundation. |
| Title Ser Add Ent |
Research reporting series. Environmental health effects research ; EPA 650/1-75/004. |
| PUB Date Free Form |
May 1975 |
| Series Title Untraced |
Environmental health effects research series ; EPA 650/1-75/004 |
| NTIS Prices |
PC A04/MF A01 |
| BIB Level |
m |
| Medium |
unmediated |
| Content |
text |
| Carrier |
volume |
| Cataloging Source |
OCLC/T |
| OCLC Time Stamp |
20200818081634 |
| Language |
eng |
| Origin |
OCLC |
| Type |
MERGE |
| OCLC Rec Leader |
02404cam 2200553Ka 45010 |
