Main Title |
Comparative Toxicities of Different Forms of Asbestos in a Cell Culture Assay. |
Author |
Reiss, Betti ;
Solomon, Sondra ;
Weisburger, John H. ;
Williams, Gary M. ;
|
CORP Author |
American Health Foundation, Valhalla, NY. Naylor Dana Inst. for Disease Prevention.;Health Effects Research Lab., Cincinnati, OH. |
Year Published |
1980 |
Report Number |
EPA-R-803998; EPA-600/J-80-282; |
Stock Number |
PB81-213084 |
Additional Subjects |
Asbestos ;
Toxicology ;
Bioassay ;
Inhibition ;
Cells(Biology) ;
Reprints ;
Cell cultures ;
Toxic substances ;
Cytotoxicity ;
NTISEPAORD
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-213084 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
23p |
Abstract |
Three forms of Union Internationale Contre le Cancer (UICC) asbestos, amosite, crocidolite, and chrysotile, were assayed for their cytotoxicity (inhibition of colony formation) in cell culture. Using embryonic human intestine-derived (I-407) and adult rat liver-derived (ARL-6) epithelial cells, the order of cytotoxicity was chrysotile>amosite>crocidolite. All three asbestos types were more toxic to I-407 than to ARL-6 cells. Chrysotile, amosite, and crocidolite were also tested for inhibition of colony formation in cultures of mouse colon-derived epithelial-like (MCE-1) cells; these cells were more resistant than the I-407 cells to all three fiber types; although similar to the ARL-6 cells in sensitivity to chrysotile and amosite, the MCE-1 cells were more sensitive to crocidolite than the ARL-6 cells. Leaching of the three asbestos forms for 3 days in sterile deionized water did not appreciably affect the cytotoxicity of I-407 or ARL-6 cells. Leaching in hydrochloric acid, however, greatly decreased the cytotoxicity of chrysotile on these cells and particularly in cultures of ARL-6 cells slightly increased the cytotoxicity of amosite and crocidolite. Leaching in deionized water altered the composition of the asbestos as revealed by release of substantial levels of Mg2+ and Ca2+ into the leaching fluid. Greater titers of these ions were released during leaching in hydrochloric acid. |
Supplementary Notes |
Pub. in Environmental Research 22, p109-129 1980. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Comparative Toxicities of Different Forms of Asbestos in a Cell Culture Assay. |
PUB Date Free Form |
c1980 |
Category Codes |
6T; 6C; 57Y; 57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
323799710 |
Origin |
NTIS |
Type |
CAT |